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Literature summary extracted from

  • Smith, E.T.; Perry, E.T.; Sears, M.B.; Johnson, D.A.
    Expression of recombinant human mast cell chymase with Asn-linked glycans in glycoengineered Pichia pastoris (2014), Protein Expr. Purif., 102, 69-75.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.39 expressed in the SuperMan5 strain of GlycoSwitch Pichia pastoris Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.39 Eglin c potent inhibitor Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.39 0.0157
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Phe-thiobenzyl ester in the presence of heparin, at pH 7.4 and 37°C Homo sapiens
3.4.21.39 0.0355
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Phe-thiobenzyl ester in the absence of heparin, at pH 7.4 and 37°C Homo sapiens
3.4.21.39 0.514
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide in the presence of heparin, at pH 7.4 and 37°C Homo sapiens
3.4.21.39 0.935
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide in the absence of heparin, at pH 7.4 and 37°C Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.39 25000
-
 x * 25000, deglycosylated recombinant enzyme, SDS-PAGE Homo sapiens
3.4.21.39 30000
-
 x * 30000, recombinant enzyme, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.39 angiotensin I + H2O Homo sapiens
-
 angiotensin II + His-Leu
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.39 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.21.39 glycoprotein
-
 Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.39 heparin Hyper M column chromatography and heparin affinity chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.39 angiotensin I + H2O
-
 Homo sapiens angiotensin II + His-Leu
-
 ?
3.4.21.39 succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide + H2O
-
 Homo sapiens succinyl-L-Ala-L-Ala-L-Pro-L-Phe + 4-nitroaniline
-
 ?
3.4.21.39 succinyl-L-Ala-L-Ala-L-Pro-L-Phe-thiobenzyl ester + H2O
-
 Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.39 ? x * 30000, recombinant enzyme, SDS-PAGE Homo sapiens
3.4.21.39 ? x * 25000, deglycosylated recombinant enzyme, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.4.21.39 mast cell chymase
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.39 2.5
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide in the presence of heparin, at pH 7.4 and 37°C Homo sapiens
3.4.21.39 3.6
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide in the absence of heparin, at pH 7.4 and 37°C Homo sapiens
3.4.21.39 12.6
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Phe-thiobenzyl ester in the absence of heparin, at pH 7.4 and 37°C Homo sapiens
3.4.21.39 17.8
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Phe-thiobenzyl ester in the presence of heparin, at pH 7.4 and 37°C Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.21.39 3.85
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide in the absence of heparin, at pH 7.4 and 37°C Homo sapiens
3.4.21.39 4.86
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide in the presence of heparin, at pH 7.4 and 37°C Homo sapiens
3.4.21.39 355
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Phe-thiobenzyl ester in the absence of heparin, at pH 7.4 and 37°C Homo sapiens
3.4.21.39 1130
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Phe-thiobenzyl ester in the presence of heparin, at pH 7.4 and 37°C Homo sapiens