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Literature summary extracted from

  • Abdul Manas, N.H.; Pachelles, S.; Mahadi, N.M.; Illias, R.M.
    The characterisation of an alkali-stable maltogenic amylase from Bacillus lehensis G1 and improved malto-oligosaccharide production by hydrolysis suppression (2014), PLoS ONE, 9, e106481.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.133 2-mercaptoethanol about 120% activity at 1% (v/v) Alkalihalobacillus lehensis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.133 expressed in Escherichia coli BL21(DE3) cells Alkalihalobacillus lehensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.133 Ca2+ about 76% residual activity at 5 mM Alkalihalobacillus lehensis
3.2.1.133 Co2+ about 13% residual activity at 5 mM Alkalihalobacillus lehensis
3.2.1.133 Cu2+ about 5% residual activity at 4 mM Alkalihalobacillus lehensis
3.2.1.133 Fe2+ about 36% residual activity at 5 mM Alkalihalobacillus lehensis
3.2.1.133 Li+ about 87% residual activity at 5 mM Alkalihalobacillus lehensis
3.2.1.133 Mg2+ about 84% residual activity at 5 mM Alkalihalobacillus lehensis
3.2.1.133 additional information not inhibited by EDTA and Tween 20 Alkalihalobacillus lehensis
3.2.1.133 Ni2+ about 2% residual activity at 5 mM Alkalihalobacillus lehensis
3.2.1.133 Pb2+ about 2% residual activity at 5 mM Alkalihalobacillus lehensis
3.2.1.133 phenylmethylsulfonyl fluoride about 2% residual activity at 1% (v/v) Alkalihalobacillus lehensis
3.2.1.133 SDS about 6% residual activity at 1% (v/v) Alkalihalobacillus lehensis
3.2.1.133 Zn2+ about 7% residual activity at 5 mM Alkalihalobacillus lehensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.133 Mn2+ about 162% activity at 5 mM Alkalihalobacillus lehensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.133 68000
-
 2 * 68000, SDS-PAGE Alkalihalobacillus lehensis
3.2.1.133 104700
-
 calculated from gel filtration Alkalihalobacillus lehensis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.133 Alkalihalobacillus lehensis X5CPN2
-
-
3.2.1.133 Alkalihalobacillus lehensis G1 X5CPN2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.133 Ni-NTA column chromatography and Superdex 200 gel filtration Alkalihalobacillus lehensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.133 alpha-cyclodextrin + H2O 80% activity compared to beta-cyclodextrin Alkalihalobacillus lehensis ?
-
 ?
3.2.1.133 amylopectin + H2O less than 2% activity compared to beta-cyclodextrin Alkalihalobacillus lehensis ?
-
 ?
3.2.1.133 amylopectin + H2O less than 2% activity compared to beta-cyclodextrin Alkalihalobacillus lehensis G1 ?
-
 ?
3.2.1.133 amylose + H2O 45% activity compared to beta-cyclodextrin Alkalihalobacillus lehensis ?
-
 ?
3.2.1.133 amylose + H2O 45% activity compared to beta-cyclodextrin Alkalihalobacillus lehensis G1 ?
-
 ?
3.2.1.133 beta-cyclodextrin + H2O 100% activity Alkalihalobacillus lehensis D-glucose + maltose + maltotriose + maltooligosaccharides
-
 ?
3.2.1.133 gamma-cyclodextrin + H2O 10% activity compared to beta-cyclodextrin Alkalihalobacillus lehensis ?
-
 ?
3.2.1.133 gamma-cyclodextrin + H2O 10% activity compared to beta-cyclodextrin Alkalihalobacillus lehensis G1 ?
-
 ?
3.2.1.133 maltotriose + H2O
-
 Alkalihalobacillus lehensis maltose + D-glucose
-
 ?
3.2.1.133 maltotriose + H2O
-
 Alkalihalobacillus lehensis G1 maltose + D-glucose
-
 ?
3.2.1.133 soluble starch + H2O 45% activity compared to beta-cyclodextrin Alkalihalobacillus lehensis ?
-
 ?
3.2.1.133 soluble starch + H2O 45% activity compared to beta-cyclodextrin Alkalihalobacillus lehensis G1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.133 homodimer 2 * 68000, SDS-PAGE Alkalihalobacillus lehensis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.133 MAG1
-
 Alkalihalobacillus lehensis
3.2.1.133 maltogenic amylase
-
 Alkalihalobacillus lehensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.133 40
-
-
 Alkalihalobacillus lehensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.133 -20 40 the enzyme retains more than 50% of its activity at 40°C and 30°C for 10 min. The half-lives of the enzyme are 239.02 h, 126.03 h and 108.30 h at -20°C, 4°C and 20°C, respectively. However, the half-lives are dramatically reduced to 0.37 h and 0.04 h at 30°C and 40°C, respectively Alkalihalobacillus lehensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.133 9.9
-
 soluble starch at pH 7.0 and 40°C Alkalihalobacillus lehensis
3.2.1.133 12.5
-
 beta-cyclodextrin at pH 7.0 and 40°C Alkalihalobacillus lehensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.133 7
-
-
 Alkalihalobacillus lehensis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.133 7 9 the enzyme is highly active and stable in a pH range from 7.0 to 9.0 Alkalihalobacillus lehensis