EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.19.36 | the desA gene, which encodes the DELTA12 desaturase of Synechocystis sp. PCC 6803, and the desB gene are introduced into Synechococcus sp. PCC 7942. Whilst the parent cyanobacterium can only desaturate membrane lipids at the DELTA9 position of fatty acids, the resultant transformant is able to desaturate fatty acids of membrane lipids at the DELTA9, DELTA12 and omega3 positions | Synechocystis sp. |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.19.36 | additional information | the desB gene in Synechocystis sp. PCC 6803 is mutated by insertion of a kanamycin-resistance gene cartridge. The resultant mutant is unable to desaturate fatty acids at the omega3 position | Synechocystis sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.19.36 | 41900 | - |
x * 41900, SDS-PAGE | Synechocystis sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.19.36 | a 1-gamma-linolenoyl 2-acyl-sn-glycerol 3-phosphate + 2 reduced ferredoxin + O2 + 2 H+ | Synechocystis sp. | - |
a 1-stearidonoyl 2-acyl-sn-glycerol 3-phosphate + 2 oxidized ferredoxin + 2 H2O | - |
? | |
1.14.19.36 | a 1-linoleoyl 2-acyl-sn-glycerol 3-phosphate + 2 reduced ferredoxin + O2 + 2 H+ | Synechocystis sp. | - |
a 1-alpha-linolenoyl 2-acyl-sn-glycerol 3-phosphate + 2 oxidized ferredoxin + 2 H2O | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.19.36 | Synechocystis sp. | Q55240 | - |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.14.19.36 | a 1-gamma-linolenoyl 2-acyl-sn-glycerol 3-phosphate + 2 reduced ferredoxin + O2 + 2 H+ = a 1-stearidonoyl 2-acyl-sn-glycerol 3-phosphate + 2 oxidized ferredoxin + 2 H2O | (1) | Synechocystis sp. | |
1.14.19.36 | a 1-linoleoyl 2-acyl-sn-glycerol 3-phosphate + 2 reduced ferredoxin + O2 + 2 H+ = a 1-alpha-linolenoyl 2-acyl-sn-glycerol 3-phosphate + 2 oxidized ferredoxin + 2 H2O | (2) | Synechocystis sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.19.36 | a 1-gamma-linolenoyl 2-acyl-sn-glycerol 3-phosphate + 2 reduced ferredoxin + O2 + 2 H+ | - |
Synechocystis sp. | a 1-stearidonoyl 2-acyl-sn-glycerol 3-phosphate + 2 oxidized ferredoxin + 2 H2O | - |
? | |
1.14.19.36 | a 1-linoleoyl 2-acyl-sn-glycerol 3-phosphate + 2 reduced ferredoxin + O2 + 2 H+ | - |
Synechocystis sp. | a 1-alpha-linolenoyl 2-acyl-sn-glycerol 3-phosphate + 2 oxidized ferredoxin + 2 H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.19.36 | ? | x * 41900, SDS-PAGE | Synechocystis sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.19.36 | DesB | - |
Synechocystis sp. |
EC Number | Organism | Comment | Expression |
---|---|---|---|
1.14.19.36 | Synechocystis sp. | the level of the desB transcript in cells grown at 22°C is 10 times higher than that in cells grown at 34°C | up |