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Literature summary extracted from

  • Liebminger, E.; Grass, J.; Jez, J.; Neumann, L.; Altmann, F.; Strasser, R.
    Myrosinases TGG1 and TGG2 from Arabidopsis thaliana contain exclusively oligomannosidic N-glycans (2012), Phytochemistry, 84, 24-30.
    View publication on PubMedView publication on EuropePMC

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.147 Arabidopsis thaliana P37702
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3.2.1.147 Arabidopsis thaliana Q9C5C2
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3.2.1.147 Arabidopsis thaliana Columbia-0 P37702
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3.2.1.147 Arabidopsis thaliana Columbia-0 Q9C5C2
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Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.147 glycoprotein all four potential N-glycosylation sites are subjected to glycosylation and are occupied by oligomannosidic structures with Man5GlcNAc2 as the main oligosaccharide, found on the majority of TGG1 glycopeptides. In addition, Man6GlcNAc2, Man7GlcNAc2 and Man8GlcNAc2 are also present on several glycosylation sites, while on Asn108 the predominant peak corresponds to Man9Glc-NAc2. TGG2 N-glycans are not altered in the mvp1 mutant Arabidopsis thaliana
3.2.1.147 glycoprotein all nine potential N-glycosylation sites are subjected to glycosylation and are occupied by oligomannosidic structures with Man5GlcNAc2 as the main oligosaccharide, found on the majority of TGG1 glycopeptides. In addition, Man6GlcNAc2, Man7GlcNAc2 and Man8GlcNAc2 are also present on several glycosylation sites, while on Asn108 the predominant peak corresponds to Man9Glc-NAc2. TGG1 from mns mutants displays incompletely processed oligomannosidic N-glycans, while TGG1 N-glycans are not altered in the mvp1 mutant Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.147 guard cell TGG1 is highly abundant in guard cells of leaves Arabidopsis thaliana
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3.2.1.147 leaf TGG1 is cell type-specific expressed in specialized myrosin cells Arabidopsis thaliana
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3.2.1.147 additional information TGG1 accumulates abnormally in mns mutants Arabidopsis thaliana
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3.2.1.147 additional information TGG2 accumulates abnormally in mns mutants Arabidopsis thaliana
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3.2.1.147 rosette leaf
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 Arabidopsis thaliana
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Synonyms

EC Number Synonyms Comment Organism
3.2.1.147 myrosinase
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 Arabidopsis thaliana
3.2.1.147 TGG1
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 Arabidopsis thaliana
3.2.1.147 TGG2
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 Arabidopsis thaliana

Expression

EC Number Organism Comment Expression
3.2.1.147 Arabidopsis thaliana TGG1 transcript levels are higher in leaves of mns1 mns2 mns3 plants up

General Information

EC Number General Information Comment Organism
3.2.1.147 additional information the enzyme is sensitive to endoglycosidase digestion Arabidopsis thaliana