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Literature summary extracted from

  • Giacobbe, S.; Vincent, F.; Faraco, V.
    Development of an improved variant of GH51 alpha-L-arabinofuranosidase from Pleurotus ostreatus by directed evolution (2014), New Biotechnol., 31, 230-236.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.55 gene poabf, screening of a genetic library after random mutagenesis using error prone PCR system, recombinant expression of wild-type and mutant enzymes in Pichia pastoris using vector pSAL4 Pleurotus ostreatus

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.55 F435Y/Y446F random mutagenesis, the mutant shows 3fold increased activity and altered substrate specificity and is active with larch arabinogalactan in contrast to the wild-type enzyme Pleurotus ostreatus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.55 Pleurotus ostreatus G0TES6 gene poabf
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.55 36.7
-
 wild-type enzyme, substrate 4-nitrophenyl-alpha-L-arabinofuranoside, pH 5.0, 40°C Pleurotus ostreatus
3.2.1.55 121.7
-
 recombinant enzyme mutant F435Y/Y446F, substrate 4-nitrophenyl-alpha-L-arabinofuranoside, pH 5.0, 40°C Pleurotus ostreatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.55 1,5-alpha-L-arabinohexaose + H2O
-
 Pleurotus ostreatus 1,5-alpha-L-arabinopentaose + L-arabinose
-
 ?
3.2.1.55 1,5-alpha-L-arabinotriose + H2O
-
 Pleurotus ostreatus 1,5-alpha-L-arabinobiose + L-arabinose
-
 ?
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Pleurotus ostreatus 4-nitrophenol + L-arabinofuranose
-
 ?
3.2.1.55 L-arabinan + H2O CM-linear arabinan Pleurotus ostreatus ?
-
 ?
3.2.1.55 larch arabinoxylan + H2O substrate of mutant F435Y/Y446F, no activity with the wild-type enzyme Pleurotus ostreatus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.55 GH51 alpha-L-arabinofuranosidase
-
 Pleurotus ostreatus
3.2.1.55 PoAbf
-
 Pleurotus ostreatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.55 40
-
-
 Pleurotus ostreatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.55 40
-
 purified wild-type and mutant enzymes, pH 5.0, loss of over 90% and 50% activity after 24 h, respectively Pleurotus ostreatus
3.2.1.55 50
-
 purified wild-type and mutant enzymes, pH 5.0, loss of 75% and 55% activity after 23 h, respectively Pleurotus ostreatus
3.2.1.55 60
-
 purified wild-type and mutant enzymes, pH 5.0, loss of 75% and 70% activity after 5 min, respectively, almost inactivation after 10 min Pleurotus ostreatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.55 5
-
-
 Pleurotus ostreatus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.55 3
-
 purified wild-type and mutant enzymes, 40°C, loss of 60-70% activity after 35 days Pleurotus ostreatus
3.2.1.55 5
-
 purified wild-type and mutant enzymes, 40°C, completely stable up to 42 days Pleurotus ostreatus
3.2.1.55 7 8 purified wild-type and mutant enzymes, 40°C, loss of 80% activity after 50 days Pleurotus ostreatus