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Literature summary extracted from

  • Ikeda, T.; Takasawa, S.; Noguchi, N.; Nata, K.; Yamauchi, A.; Takahashi, I.; Yoshikawa, T.; Sugawara, A.; Yonekura, H.; Okamoto, H.
    Identification of a major enzyme for the synthesis and hydrolysis of cyclic ADP-ribose in amphibian cells and evolutional conservation of the enzyme from human to invertebrate (2012), Mol. Cell. Biochem., 366, 69-80.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.2.6 gene cd38, eight exons, DNA and amino acid sequence determination and analysis, sequence comparison to the human enzyme, phylogenetic tree, expression in COS-7 cells Xenopus laevis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.2.6 membrane most frog enzyme (81%) is distributed in the membrane fraction (5% in mitochondrial fraction and 14% in microsomal fraction) Xenopus laevis 16020
-
3.2.2.6 microsome
-
 Xenopus laevis
-
-
3.2.2.6 mitochondrion mitochondrial membrane Xenopus laevis 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.2.6 28970
-
 x * 45000, SDS-PAGE, x * 28970, sequence calculation Xenopus laevis
3.2.2.6 45000
-
 x * 45000, SDS-PAGE, x * 28970, sequence calculation Xenopus laevis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.6 cyclic ADP-ribose + H2O Xenopus laevis
-
 ADP-D-ribose
-
 ?
3.2.2.6 additional information Xenopus laevis frog CD38 enzyme shows NAD+ glycohydrolase, ADP-ribosyl cyclase, and cyclic ADP-ribosyl hydrolase activities ?
-
 ?
3.2.2.6 NAD+ Xenopus laevis
-
 cyclic ADP-ribose + nicotinamide
-
 ?
3.2.2.6 NAD+ + H2O Xenopus laevis overall reaction ADP-D-ribose + nicotinamide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.6 Xenopus laevis H7C831 gene cd38
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.2.6 glycoprotein
-
 Xenopus laevis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.2.6 brain
-
 Xenopus laevis
-
3.2.2.6 liver
-
 Xenopus laevis
-
3.2.2.6 additional information the enzyme is ubiquitously expressed in various tissues Xenopus laevis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.2.6 0.0604
-
 NAD+-glycohydrolase activityin crude cell extract, pH 7.4, 37°C Xenopus laevis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.6 cyclic ADP-ribose + H2O
-
 Xenopus laevis ADP-D-ribose
-
 ?
3.2.2.6 additional information frog CD38 enzyme shows NAD+ glycohydrolase, ADP-ribosyl cyclase, and cyclic ADP-ribosyl hydrolase activities Xenopus laevis ?
-
 ?
3.2.2.6 NAD+
-
 Xenopus laevis cyclic ADP-ribose + nicotinamide
-
 ?
3.2.2.6 NAD+ + H2O overall reaction Xenopus laevis ADP-D-ribose + nicotinamide
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.2.6 ? x * 45000, SDS-PAGE, x * 28970, sequence calculation Xenopus laevis

Synonyms

EC Number Synonyms Comment Organism
3.2.2.6 CD38
-
 Xenopus laevis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.2.6 37
-
 assay at Xenopus laevis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.6 7.4
-
 assay at Xenopus laevis

General Information

EC Number General Information Comment Organism
3.2.2.6 evolution the CD38-cADPR signaling system is conserved during vertebrate evolution, phylogenetic tree Xenopus laevis
3.2.2.6 physiological function the enzyme is CD38-cADPR signaling system in frog cells Xenopus laevis