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Literature summary extracted from

  • Chahed, H.; Ezzine, A.; Mlouka, A.B.; Hardouin, J.; Jouenne, T.; Marzouki, M.N.
    Biochemical characterization, molecular cloning, and structural modeling of an interesting beta-1,4-glucanase from Sclerotinia sclerotiorum (2014), Mol. Biotechnol., 56, 340-350.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 DNA and amino acid sequence determination and analysis Sclerotinia sclerotiorum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.4 extracellular
-
 Sclerotinia sclerotiorum
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 27000
-
 x * 27000, SDS-PAGE Sclerotinia sclerotiorum
3.2.1.4 33297
-
 x * 33297, sequence calculation, x * 34000, SDS-PAGE Sclerotinia sclerotiorum
3.2.1.4 34000
-
 x * 33297, sequence calculation, x * 34000, SDS-PAGE Sclerotinia sclerotiorum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Sclerotinia sclerotiorum A7E948
-
-
3.2.1.4 Sclerotinia sclerotiorum P21833
-
-
3.2.1.4 Sclerotinia sclerotiorum P21834
-
-
3.2.1.4 Sclerotinia sclerotiorum 1980 A7E948
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 carboxymethyl cellulose + H2O release of reducing sugar, degradation Sclerotinia sclerotiorum ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O release of reducing sugar, degradation Sclerotinia sclerotiorum 1980 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 ? x * 27000, SDS-PAGE Sclerotinia sclerotiorum
3.2.1.4 ? x * 33297, sequence calculation, x * 34000, SDS-PAGE Sclerotinia sclerotiorum
3.2.1.4 More the enzyme contains two domains, a core domain having a high similarity with endoglucanases family 5 and a cellulose-binding domain having similarities with those of exo-type cellulases of family 1, linked together by a serine-threonine-rich region, peptide mapping, mass spectrometric analysis Sclerotinia sclerotiorum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 CMCase
-
 Sclerotinia sclerotiorum
3.2.1.4 endo-beta-1,4-glucanase
-
 Sclerotinia sclerotiorum
3.2.1.4 endo-beta-D-1,4-glucanohydrolase
-
 Sclerotinia sclerotiorum
3.2.1.4 ENDO1
-
 Sclerotinia sclerotiorum
3.2.1.4 ENDO2
-
 Sclerotinia sclerotiorum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 50
-
-
 Sclerotinia sclerotiorum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.4 25 70 activity range, profile overview Sclerotinia sclerotiorum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 5
-
-
 Sclerotinia sclerotiorum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.4 4.5 6.5 activity range, profile overview Sclerotinia sclerotiorum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.4 Sclerotinia sclerotiorum sequence calculation
-
 6.43

Expression

EC Number Organism Comment Expression
3.2.1.4 Sclerotinia sclerotiorum extracellular endo-beta-1,4-glucanase isozyme Endo1 is induced by carboxymethyl cellulose up
3.2.1.4 Sclerotinia sclerotiorum extracellular endo-beta-1,4-glucanase isozyme Endo2 is induced by carboxymethyl cellulose up

General Information

EC Number General Information Comment Organism
3.2.1.4 evolution isozyme Endo1 belongs to the glycosyl hydrolase family 45, GH45 Sclerotinia sclerotiorum
3.2.1.4 additional information protein structure homology molecular modeling and three-dimensional structure comparison, overview Sclerotinia sclerotiorum