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Literature summary extracted from

  • Kang, J.; Ju, H.; Ju, J.; Sohn, W.; Kim, T.; Bahk, Y.; Hong, S.; Na, B.
    Comparative biochemical and functional properties of two leucine aminopeptidases of Clonorchis sinensis (2012), Mol. Biochem. Parasitol., 182, 17-26.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.1 expressed in Escherichia coli as a His-tagged fusion protein Clonorchis sinensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.1 amastatin
-
 Clonorchis sinensis
3.4.11.1 bestatin
-
 Clonorchis sinensis
3.4.11.1 EDTA
-
 Clonorchis sinensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.1 0.035
-
 L-Leu-4-nitroanilide pH 8, 20°C Clonorchis sinensis
3.4.11.1 0.036
-
 L-Leu-4-nitroanilide pH 8, 20°C Clonorchis sinensis
3.4.11.1 0.053
-
 L-Met-4-nitroanilide pH 8, 20°C Clonorchis sinensis
3.4.11.1 0.0597
-
 L-Met-4-nitroanilide pH 8, 20°C Clonorchis sinensis
3.4.11.1 0.0842
-
 L-Phe-4-nitroanilide pH 8, 20°C Clonorchis sinensis
3.4.11.1 0.091
-
 L-Phe-4-nitroanilide pH 8, 20°C Clonorchis sinensis
3.4.11.1 0.094
-
 L-Lys-4-nitroanilide pH 8, 20°C Clonorchis sinensis
3.4.11.1 0.103
-
 L-Lys-4-nitroanilide pH 8, 20°C Clonorchis sinensis
3.4.11.1 0.185
-
 L-Ala-4-nitroanilide pH 8, 20°C Clonorchis sinensis
3.4.11.1 0.198
-
 L-Thr-4-nitroanilide pH 8, 20°C Clonorchis sinensis
3.4.11.1 0.202
-
 L-Ala-4-nitroanilide pH 8, 20°C Clonorchis sinensis
3.4.11.1 0.204
-
 L-Thr-4-nitroanilide pH 8, 20°C Clonorchis sinensis
3.4.11.1 0.216
-
 L-Pro-4-nitroanilide pH 8, 20°C Clonorchis sinensis
3.4.11.1 0.239
-
 L-Pro-4-nitroanilide pH 8, 20°C Clonorchis sinensis
3.4.11.1 0.261
-
 L-Ile-4-nitroanilide pH 8, 20°C Clonorchis sinensis
3.4.11.1 0.372
-
 L-Val-4-nitroanilide pH 8, 20°C Clonorchis sinensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.1 Mg2+ enhances activity Clonorchis sinensis
3.4.11.1 Mn2+ enhances activity Clonorchis sinensis
3.4.11.1 Zn2+ enhances activity Clonorchis sinensis

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.1 Clonorchis sinensis C6FWG9
-
-
3.4.11.1 Clonorchis sinensis C6FWH0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.1 using Ni-NTA chromatography Clonorchis sinensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.1 epithelial cell co-localizes with CsLAP1 to the intestinal epithelial cells of the parasite Clonorchis sinensis
-
3.4.11.1 epithelial cell co-localizes with CsLAP2 to the intestinal epithelial cells of the parasite Clonorchis sinensis
-
3.4.11.1 gastrodermis co-localizes with CsLAP1 to the gastrodermis of the parasite Clonorchis sinensis
-
3.4.11.1 gastrodermis co-localizes with CsLAP2 to the gastrodermis of the parasite Clonorchis sinensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.1 L-Ala-4-nitroanilide + H2O
-
 Clonorchis sinensis L-Ala + 4-nitroaniline
-
 ?
3.4.11.1 L-Ile-4-nitroanilide + H2O
-
 Clonorchis sinensis L-Ile + 4-nitroaniline
-
 ?
3.4.11.1 L-Leu-4-nitroanilide + H2O
-
 Clonorchis sinensis L-Leu + 4-nitroaniline
-
 ?
3.4.11.1 L-Lys-4-nitroanilide + H2O
-
 Clonorchis sinensis L-Lys + 4-nitroaniline
-
 ?
3.4.11.1 L-Met-4-nitroanilide + H2O
-
 Clonorchis sinensis L-Met + 4-nitroaniline
-
 ?
3.4.11.1 L-Phe-4-nitroanilide + H2O
-
 Clonorchis sinensis L-Phe + 4-nitroaniline
-
 ?
3.4.11.1 L-Pro-4-nitroanilide + H2O
-
 Clonorchis sinensis L-Pro + 4-nitroaniline
-
 ?
3.4.11.1 L-Thr-4-nitroanilide + H2O
-
 Clonorchis sinensis L-Thr + 4-nitroaniline
-
 ?
3.4.11.1 L-Val-4-nitroanilide + H2O
-
 Clonorchis sinensis L-Val + 4-nitroaniline
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.11.1 CsLAP1
-
 Clonorchis sinensis
3.4.11.1 CsLAP2
-
 Clonorchis sinensis
3.4.11.1 leucine aminopeptidase
-
 Clonorchis sinensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.1 20
-
 assay at Clonorchis sinensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.1 0.01
-
 L-Val-4-nitroanilide pH 8, 20°C Clonorchis sinensis
3.4.11.1 0.01
-
 L-Ile-4-nitroanilide pH 8, 20°C Clonorchis sinensis
3.4.11.1 0.01
-
 L-Thr-4-nitroanilide pH 8, 20°C Clonorchis sinensis
3.4.11.1 0.02
-
 L-Pro-4-nitroanilide pH 8, 20°C Clonorchis sinensis
3.4.11.1 0.02
-
 L-Thr-4-nitroanilide pH 8, 20°C Clonorchis sinensis
3.4.11.1 0.04
-
 L-Phe-4-nitroanilide pH 8, 20°C Clonorchis sinensis
3.4.11.1 0.05
-
 L-Lys-4-nitroanilide pH 8, 20°C Clonorchis sinensis
3.4.11.1 0.05
-
 L-Phe-4-nitroanilide pH 8, 20°C Clonorchis sinensis
3.4.11.1 0.07
-
 L-Lys-4-nitroanilide pH 8, 20°C Clonorchis sinensis
3.4.11.1 0.12
-
 L-Ala-4-nitroanilide pH 8, 20°C Clonorchis sinensis
3.4.11.1 0.18
-
 L-Met-4-nitroanilide pH 8, 20°C Clonorchis sinensis
3.4.11.1 0.2
-
 L-Met-4-nitroanilide pH 8, 20°C Clonorchis sinensis
3.4.11.1 0.23
-
 L-Leu-4-nitroanilide pH 8, 20°C Clonorchis sinensis
3.4.11.1 0.27
-
 L-Leu-4-nitroanilide pH 8, 20°C Clonorchis sinensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.1 8
-
 assay at Clonorchis sinensis

Expression

EC Number Organism Comment Expression
3.4.11.1 Clonorchis sinensis expression at transcription levels increases gradually in accordance with the maturation of the parasite up

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.11.1 0.027
-
 L-Val-4-nitroanilide pH 8, 20°C Clonorchis sinensis
3.4.11.1 0.038
-
 L-Ile-4-nitroanilide pH 8, 20°C Clonorchis sinensis
3.4.11.1 0.049
-
 L-Thr-4-nitroanilide pH 8, 20°C Clonorchis sinensis
3.4.11.1 0.083
-
 L-Pro-4-nitroanilide pH 8, 20°C Clonorchis sinensis
3.4.11.1 0.092
-
 L-Pro-4-nitroanilide pH 8, 20°C Clonorchis sinensis
3.4.11.1 0.1
-
 L-Thr-4-nitroanilide pH 8, 20°C Clonorchis sinensis
3.4.11.1 0.44
-
 L-Phe-4-nitroanilide pH 8, 20°C Clonorchis sinensis
3.4.11.1 0.53
-
 L-Lys-4-nitroanilide pH 8, 20°C Clonorchis sinensis
3.4.11.1 0.59
-
 L-Ala-4-nitroanilide pH 8, 20°C Clonorchis sinensis
3.4.11.1 0.59
-
 L-Phe-4-nitroanilide pH 8, 20°C Clonorchis sinensis
3.4.11.1 0.6
-
 L-Ala-4-nitroanilide pH 8, 20°C Clonorchis sinensis
3.4.11.1 0.68
-
 L-Lys-4-nitroanilide pH 8, 20°C Clonorchis sinensis
3.4.11.1 3
-
 L-Met-4-nitroanilide pH 8, 20°C Clonorchis sinensis
3.4.11.1 3.7
-
 L-Met-4-nitroanilide pH 8, 20°C Clonorchis sinensis
3.4.11.1 6.3
-
 L-Leu-4-nitroanilide pH 8, 20°C Clonorchis sinensis
3.4.11.1 7.7
-
 L-Leu-4-nitroanilide pH 8, 20°C Clonorchis sinensis