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Literature summary extracted from

  • Kishor, C.; Gumpena, R.; Reddi, R.; Addlagatta, A.
    Structural studies of Enterococcus faecalis methionine aminopeptidase and design of microbe specific 2,2'-bipyridine based inhibitors (2012), MedChemComm, 3, 1406-1412.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.18 expression in Escherichia coli Enterococcus faecalis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.11.18 apo-form of enzyme and modeling of inhibitor 2,2'-bipyridine. One of the rings of 2,2'-bipyridine that points into the S1 pocket is surrounded by residues Tyr60, Tyr63, Trp218, and Cys68, while the other ring points towards the surface of the protein that does not make any contact with the protein, except with the metal ion Enterococcus faecalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.18 2,2'-bipyridine maximum inhibition is observed in the presence of CoCl2 rather than other metal ions Enterococcus faecalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.18 0.000247
-
 L-methionine 4-nitroanilide pH 7.5, 30°C Enterococcus faecalis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.18 Co2+ absolutely required, maximal enzyme activity is observed at 3.0 molar equivalents Enterococcus faecalis
3.4.11.18 Mn2+ may substitute for Co2+, about 50% of cobalt activation Enterococcus faecalis
3.4.11.18 Ni2+ may substitute for Co2+, maximal enzyme activity is observed at 10.0 molar equivalents Enterococcus faecalis
3.4.11.18 Zn2+ may substitute for Co2+, maximal enzyme activity is observed at 10.0 molar equivalents. Activity with Zn2+ is not dependent on pH Enterococcus faecalis

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.18 Enterococcus faecalis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.18 L-methionine 4-nitroanilide + H2O
-
 Enterococcus faecalis L-Met + 4-nitroaniline
-
 ?
3.4.11.18 additional information no substrates: Gly 4-nitroanilide , Ala 4-nitroanilide, Ser 7-amido-4-methyl-coumarin, Leu 4-nitroanilide, Glu 4-nitroanilide, Lys 4-nitroanilide and Arg 4-nitroanilide Enterococcus faecalis ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.18 0.0043
-
 L-methionine 4-nitroanilide pH 7.5, 30°C Enterococcus faecalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.18 7.5
-
 highest activity in presence of Co2+, Mn2+, Ni2+ Enterococcus faecalis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.4.11.18 0.000667
-
 pH 7.5, 30°C Enterococcus faecalis 2,2'-bipyridine