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Literature summary extracted from

  • Farhadian, S.; Asoodeh, A.; Lagzian, M.
    Purification, biochemical characterization and structural modeling of a potential htrA-like serine protease from Bacillus subtilis DR8806 (2015), J. Mol. Catal. B, 115, 51-58.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.21.107 modeling of 3D structure Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.107 Hg2+ 1 mM, complete inhibition Bacillus subtilis
3.4.21.107 PMSF 5 mM, 60% residual activity, 10 mM, no residual activity Bacillus subtilis
3.4.21.107 Sodium dodecyl sulfate 1 mM, 61% resiudal activity Bacillus subtilis
3.4.21.107 Triton X-100 5 mM, 71% residual activity Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.107 Ca2+ 10 mM, up to 129% stimulation of the protease activity Bacillus subtilis
3.4.21.107 Fe2+ 10 mM, up to 112% stimulation of the protease activity Bacillus subtilis
3.4.21.107 K+ 10 mM, up to 134% stimulation of the protease activity Bacillus subtilis
3.4.21.107 Mg2+ 10 mM, up to 128% stimulation of the protease activity Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.107 37000
-
 x * 37000, SDS-PAGE Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.107 Bacillus subtilis
-
-
-
3.4.21.107 Bacillus subtilis DR8806
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.107
-
 Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.107 79.3
-
 pH 8.0, 37°C Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.107 Bovine serum albumin + H2O 85% of the activity with casein Bacillus subtilis ?
-
 ?
3.4.21.107 Bovine serum albumin + H2O 85% of the activity with casein Bacillus subtilis DR8806 ?
-
 ?
3.4.21.107 casein + H2O
-
 Bacillus subtilis ?
-
 ?
3.4.21.107 casein + H2O
-
 Bacillus subtilis DR8806 ?
-
 ?
3.4.21.107 Gelatin + H2O 20% of the activity with casein Bacillus subtilis ?
-
 ?
3.4.21.107 Gelatin + H2O 20% of the activity with casein Bacillus subtilis DR8806 ?
-
 ?
3.4.21.107 N-acetyl-L-tyrosine ethyl ester + H2O 51% of the activity with casein Bacillus subtilis N-acetyl-L-tyrosine + ethanol
-
 ?
3.4.21.107 N-acetyl-L-tyrosine ethyl ester + H2O 51% of the activity with casein Bacillus subtilis DR8806 N-acetyl-L-tyrosine + ethanol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.107 ? x * 37000, SDS-PAGE Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.107 45
-
-
 Bacillus subtilis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.21.107 20 85 at least 50% of maximum activity Bacillus subtilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.107 50
-
 1 h, 70% residual activity Bacillus subtilis
3.4.21.107 70
-
 1 h, 30% residual activity Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.107 8
-
-
 Bacillus subtilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.21.107 5 10
-
 Bacillus subtilis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.21.107 Bacillus subtilis isoeletric focusing
-
 6.6