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Literature summary extracted from

  • Stressler, T.; Eisele, T.; Kranz, B.; Fischer, L.
    PepX from Lactobacillus helveticus: Automated multi-step purification and determination of kinetic parameters with original tripeptide substrates (2014), J. Mol. Catal. B, 108, 103-110.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.14.11 0.12
-
 L-Tyr-L-Pro-L-Ala pH 6.5, 37°C Lactobacillus helveticus
3.4.14.11 0.14
-
 L-Ala-L-Pro-L-Arg pH 6.5, 37°C Lactobacillus helveticus
3.4.14.11 0.17
-
 L-Ala-L-Pro-L-Tyr pH 6.5, 37°C Lactobacillus helveticus
3.4.14.11 0.24
-
 L-Arg-L-Pro-L-Ala pH 6.5, 37°C Lactobacillus helveticus
3.4.14.11 0.53
-
 L-Ala-L-Pro-L-Ser pH 6.5, 37°C Lactobacillus helveticus
3.4.14.11 0.54
-
 L-Ser-L-Pro-L-Ala pH 6.5, 37°C Lactobacillus helveticus
3.4.14.11 1.23
-
 L-Asp-L-Pro-L-Ala pH 6.5, 37°C Lactobacillus helveticus
3.4.14.11 1.53
-
 L-Ala-L-Pro 4-nitroanilide pH 6.5, 37°C Lactobacillus helveticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.14.11 90000
-
 x * 90000, SDS-PAGE Lactobacillus helveticus

Organism

EC Number Organism UniProt Comment Textmining
3.4.14.11 Lactobacillus helveticus S4TJ23
-
-
3.4.14.11 Lactobacillus helveticus ATCC 12046 S4TJ23
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.14.11
-
 Lactobacillus helveticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.14.11 22.2
-
 pH 6.5, 37°C Lactobacillus helveticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.14.11 L-Ala-L-Pro 4-nitroanilide + H2O
-
 Lactobacillus helveticus L-Ala-L-Pro + 4-nitroaniline
-
 ?
3.4.14.11 L-Ala-L-Pro 4-nitroanilide + H2O
-
 Lactobacillus helveticus ATCC 12046 L-Ala-L-Pro + 4-nitroaniline
-
 ?
3.4.14.11 L-Ala-L-Pro-L-Arg + H2O
-
 Lactobacillus helveticus L-Ala-L-Pro + L-Arg
-
 ?
3.4.14.11 L-Ala-L-Pro-L-Arg + H2O
-
 Lactobacillus helveticus ATCC 12046 L-Ala-L-Pro + L-Arg
-
 ?
3.4.14.11 L-Ala-L-Pro-L-Ser + H2O
-
 Lactobacillus helveticus L-Ala-L-Pro + L-Ser
-
 ?
3.4.14.11 L-Ala-L-Pro-L-Ser + H2O
-
 Lactobacillus helveticus ATCC 12046 L-Ala-L-Pro + L-Ser
-
 ?
3.4.14.11 L-Ala-L-Pro-L-Tyr + H2O
-
 Lactobacillus helveticus L-Ala-L-Pro + L-Tyr
-
 ?
3.4.14.11 L-Ala-L-Pro-L-Tyr + H2O
-
 Lactobacillus helveticus ATCC 12046 L-Ala-L-Pro + L-Tyr
-
 ?
3.4.14.11 L-Arg-L-Pro-L-Ala + H2O
-
 Lactobacillus helveticus L-Arg-L-Pro + L-Ala
-
 ?
3.4.14.11 L-Asp-L-Pro-L-Ala + H2O
-
 Lactobacillus helveticus L-Asp-L-Pro + L-Ala
-
 ?
3.4.14.11 L-Ser-L-Pro-L-Ala + H2O
-
 Lactobacillus helveticus L-Ser-L-Pro + L-Ala
-
 ?
3.4.14.11 L-Tyr-L-Pro-L-Ala + H2O
-
 Lactobacillus helveticus L-Tyr-L-Pro + L-Ala
-
 ?
3.4.14.11 additional information no substrate inhibition is determined Lactobacillus helveticus ?
-
 ?
3.4.14.11 additional information no substrate inhibition is determined Lactobacillus helveticus ATCC 12046 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.14.11 ? x * 90000, SDS-PAGE Lactobacillus helveticus

Synonyms

EC Number Synonyms Comment Organism
3.4.14.11 PepX
-
 Lactobacillus helveticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.14.11 50
-
-
 Lactobacillus helveticus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.14.11 43.5
-
 L-Ala-L-Pro-L-Tyr pH 6.5, 37°C Lactobacillus helveticus
3.4.14.11 57.2
-
 L-Ala-L-Pro-L-Arg pH 6.5, 37°C Lactobacillus helveticus
3.4.14.11 95.9
-
 L-Ala-L-Pro-L-Ser pH 6.5, 37°C Lactobacillus helveticus
3.4.14.11 133
-
 L-Arg-L-Pro-L-Ala pH 6.5, 37°C Lactobacillus helveticus
3.4.14.11 145
-
 L-Tyr-L-Pro-L-Ala pH 6.5, 37°C Lactobacillus helveticus
3.4.14.11 199
-
 L-Asp-L-Pro-L-Ala pH 6.5, 37°C Lactobacillus helveticus
3.4.14.11 233
-
 L-Ser-L-Pro-L-Ala pH 6.5, 37°C Lactobacillus helveticus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.14.11 162
-
 L-Asp-L-Pro-L-Ala pH 6.5, 37°C Lactobacillus helveticus
3.4.14.11 181
-
 L-Ala-L-Pro-L-Ser pH 6.5, 37°C Lactobacillus helveticus
3.4.14.11 256
-
 L-Ala-L-Pro-L-Tyr pH 6.5, 37°C Lactobacillus helveticus
3.4.14.11 409
-
 L-Ala-L-Pro-L-Arg pH 6.5, 37°C Lactobacillus helveticus
3.4.14.11 431
-
 L-Ser-L-Pro-L-Ala pH 6.5, 37°C Lactobacillus helveticus
3.4.14.11 552
-
 L-Arg-L-Pro-L-Ala pH 6.5, 37°C Lactobacillus helveticus
3.4.14.11 1210
-
 L-Tyr-L-Pro-L-Ala pH 6.5, 37°C Lactobacillus helveticus