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Literature summary extracted from
- Screening, purification, and characterization of an extracellular prolyl oligopeptidase from Coprinopsis clastophylla (2012), J. Microbiol., 50, 652-659.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.26 | 3,4-dichloroisocoumarin | - |
Coprinopsis clastophylla |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.26 | 4-chloromercuribenzoate | 14% residual activity at 0.2 mM | Coprinopsis clastophylla | |
| 3.4.21.26 | 4-toluenesulfonamide | 98% residual activity at 0.2 mM | Coprinopsis clastophylla | |
| 3.4.21.26 | Co2+ | 48% residual activity at 0.2 mM | Coprinopsis clastophylla | |
| 3.4.21.26 | Cu2+ | 13% residual activity at 0.2 mM | Coprinopsis clastophylla | |
| 3.4.21.26 | diisopropyl fluorophosphate | 13% residual activity at 0.2 mM | Coprinopsis clastophylla | |
| 3.4.21.26 | EDTA | 93% residual activity at 0.2 mM | Coprinopsis clastophylla | |
| 3.4.21.26 | Hg2+ | complete inhibition at 0.2 mM | Coprinopsis clastophylla | |
| 3.4.21.26 | iodoacetamide | 87% residual activity at 0.2 mM | Coprinopsis clastophylla | |
| 3.4.21.26 | Mn2+ | 93% residual activity at 0.2 mM | Coprinopsis clastophylla | |
| 3.4.21.26 | N-ethylmaleimide | 50% residual activity at 0.2 mM | Coprinopsis clastophylla | |
| 3.4.21.26 | Ni2+ | 86% residual activity at 0.2 mM | Coprinopsis clastophylla | |
| 3.4.21.26 | o-phenanthroline | 79% residual activity at 0.2 mM | Coprinopsis clastophylla | |
| 3.4.21.26 | Sn2+ | 87% residual activity at 0.2 mM | Coprinopsis clastophylla | |
| 3.4.21.26 | Zn2+ | 61% residual activity at 0.2 mM | Coprinopsis clastophylla | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.26 | 1.74 | - |
benzyloxycarbonyl-Gly-L-Pro-7-amido-4-methylcoumarin | at pH 8.0 and 37°C | Coprinopsis clastophylla | |
| 3.4.21.26 | 3.42 | - |
benzyloxycarbonyl-Gly-L-Pro-4-nitroanilide | at pH 8.0 and 37°C | Coprinopsis clastophylla | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.26 | Fe2+ | activates | Coprinopsis clastophylla | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.26 | 84000 | - |
x * 84000, SDS-PAGE | Coprinopsis clastophylla |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.26 | Coprinopsis clastophylla | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.21.26 | ammonium sulfate precipitation, phenyl Sepharose column chromatography, Sephacryl S-200 gel filtration, hydroxyapatite column chromatography, and Resource Q column chromatography | Coprinopsis clastophylla |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.26 | 0.18 | - |
supernatant, using benzyloxycarbonyl-Gly-L-Pro-4-nitroanilide as substrate, at pH 8.0 and 37°C | Coprinopsis clastophylla |
| 3.4.21.26 | 52.1 | - |
after 285fold purification, using benzyloxycarbonyl-Gly-L-Pro-4-nitroanilide as substrate, at pH 8.0 and 37°C | Coprinopsis clastophylla |
| 3.4.21.26 | 61.3 | - |
after 285fold purification, using benzyloxycarbonyl-Gly-L-Pro-7-amido-4-methylcoumarin as substrate, at pH 8.0 and 37°C | Coprinopsis clastophylla |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.26 | benzyloxycarbonyl-Gly-L-Pro-4-nitroanilide + H2O | - |
Coprinopsis clastophylla | benzyloxycarbonyl-Gly-L-Pro + 4-nitroaniline | - |
? | |
| 3.4.21.26 | benzyloxycarbonyl-Gly-L-Pro-7-amido-4-methylcoumarin + H2O | - |
Coprinopsis clastophylla | benzyloxycarbonyl-Gly-L-Pro + 7-amino-4-methylcoumarin | - |
? | |
| 3.4.21.26 | additional information | no activity toward L-Pro-4-nitroanilide, Gly-L-Pro-4-nitroanilide, Z-Pro-nitrophenyl ester, Z-Gly-Gly-nitrophenyl ester, Z-Phe-Arg-7-amido-4-methylcoumarin, Z-Leu-Leu-Glu-7-amido-4-methylcoumarin, Z-Val-Lys-Met-7-amido-4-methylcoumarin, Z-Leu-Leu-Leu-7-amido-4-methylcoumarin, and Z-Tyr-Val-Ala-Asp-4-nitroanilide | Coprinopsis clastophylla | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.21.26 | ? | x * 84000, SDS-PAGE | Coprinopsis clastophylla |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.21.26 | post-proline cleaving endopeptidase | - |
Coprinopsis clastophylla |
| 3.4.21.26 | proline-specific endopeptidase | - |
Coprinopsis clastophylla |
| 3.4.21.26 | prolyl endopeptidase | - |
Coprinopsis clastophylla |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.26 | 37 | - |
- |
Coprinopsis clastophylla |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.26 | 25 | 37 | the enzyme is active over a temperature range of 25-37°C | Coprinopsis clastophylla |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.26 | 45 | - |
the enzyme retains about 20% of its activity after incubation at 45°C for 30 min | Coprinopsis clastophylla |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.26 | 26.6 | - |
benzyloxycarbonyl-Gly-L-Pro-7-amido-4-methylcoumarin | at pH 8.0 and 37°C | Coprinopsis clastophylla | |
| 3.4.21.26 | 162 | - |
benzyloxycarbonyl-Gly-L-Pro-4-nitroanilide | at pH 8.0 and 37°C | Coprinopsis clastophylla | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.26 | 8 | - |
- |
Coprinopsis clastophylla |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.26 | 6 | 9 | the activity of the enzyme is reduced by 50% and 60% at pH 6.0 and 9.0, respectively, compared to its maximal activity | Coprinopsis clastophylla |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.4.21.26 | Coprinopsis clastophylla | isoelectric focusing | - |
5.2 |
IC50 Value
| EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.26 | 0.2 | - |
at pH 8.0 and 37°C | Coprinopsis clastophylla | N-ethylmaleimide | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.21.26 | 15.1 | - |
benzyloxycarbonyl-Gly-L-Pro-7-amido-4-methylcoumarin | at pH 8.0 and 37°C | Coprinopsis clastophylla | |
| 3.4.21.26 | 47.4 | - |
benzyloxycarbonyl-Gly-L-Pro-4-nitroanilide | at pH 8.0 and 37°C | Coprinopsis clastophylla | |
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