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Literature summary extracted from

  • Woo, M.; Kim, H.; Lee, E.
    Development and characterization of recombinant whole cells expressing the soluble epoxide hydrolase of Danio rerio and its variant for enantioselective resolution of racemic styrene oxides (2012), J. Indust. Eng. Chem., 18, 384-391.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.3.2.10 expressed in Escherichia coli BL21(DE3) cells Danio rerio

Protein Variants

EC Number Protein Variants Comment Organism
3.3.2.10 E88R/T102A/M368I/K398E/G412D the variant possesses (S)-styrene oxide-preferred hydrolytic activity Danio rerio

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.3.2.10 3.47
-
 (R)-styrene oxide wild type enzyme, at pH 6.0 and 10°C Danio rerio
3.3.2.10 7.85
-
 (S)-styrene oxide wild type enzyme, at pH 6.0 and 10°C Danio rerio
3.3.2.10 7.95
-
 (R)-styrene oxide mutant enzyme E88R/T102A/M368I/K398E/G412D, at pH 6.0 and 10°C Danio rerio
3.3.2.10 8.33
-
 (S)-styrene oxide mutant enzyme E88R/T102A/M368I/K398E/G412D, at pH 6.0 and 10°C Danio rerio

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.3.2.10 soluble
-
 Danio rerio
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.3.2.10 64800
-
 x * 64800, SDS-PAGE Danio rerio

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.3.2.10 (R)-styrene oxide + H2O Danio rerio
-
 (R)-styrene glycol
-
 ?
3.3.2.10 (S)-styrene oxide + H2O Danio rerio the enzyme possesses (S)-styrene oxide-preferred hydrolytic activity (S)-styrene glycol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.3.2.10 Danio rerio Q5PRC6
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.3.2.10 (R)-styrene oxide + H2O
-
 Danio rerio (R)-styrene glycol
-
 ?
3.3.2.10 (S)-styrene oxide + H2O the enzyme possesses (S)-styrene oxide-preferred hydrolytic activity Danio rerio (S)-styrene glycol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.3.2.10 ? x * 64800, SDS-PAGE Danio rerio

Synonyms

EC Number Synonyms Comment Organism
3.3.2.10 EPHX2
-
 Danio rerio
3.3.2.10 SEH
-
 Danio rerio

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.3.2.10 6
-
-
 Danio rerio