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Literature summary extracted from

  • Li, Z.; Roccatano, D.; Lorenz, M.; Martinez, R.; Schwaneberg, U.
    Insights on activity and stability of subtilisin E towards guanidinium chloride and sodium dodecylsulfate (2014), J. Biotechnol., 169, 87-94.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.62 gene aprE, recombinant expression of wild-type and mutant enzymes in Bacillus subtilis strain WB600 and secretion to the culture medium Bacillus subtilis

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.62 N218S site-directed mutagenesis, the mutant shows increased activity and stability in the presence of GdmCl or SDS compared to the wild-type enzyme Bacillus subtilis
3.4.21.62 S62I/A153V/G166S/I205V site-directed mutagenesis, the mutant shows increased activity and stability in the presence of GdmCl or SDS compared to the wild-type enzyme Bacillus subtilis
3.4.21.62 S62I/A153V/G166S/I205V/N218S/T224A site-directed mutagenesis, the mutant shows high activity and stability in the presence of GdmCl or SDS compared to the wild-type enzyme Bacillus subtilis
3.4.21.62 S62I/A153V/G166S/T224A/T240S site-directed mutagenesis, the mutant shows high activity and stability in the presence of GdmCl or SDS compared to the wild-type enzyme Bacillus subtilis
3.4.21.62 S62I/A153V/G166S/T224A/T240S site-directed mutagenesis, the mutant shows increased activity and stability in the presence of GdmCl or SDS compared to the wild-type enzyme Bacillus subtilis
3.4.21.62 T224A site-directed mutagenesis, the mutant shows increased activity and stability in the presence of GdmCl or SDS compared to the wild-type enzyme Bacillus subtilis

General Stability

EC Number General Stability Organism
3.4.21.62 stability of wild-type an dmutant enzymes in the presence of GdmCl or SDS, overview Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.62 guanidinium hydrochloride
-
 Bacillus subtilis
3.4.21.62 SDS
-
 Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.62 Bacillus subtilis P04189 gene aprE
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.62 recombinant wild-type and mutant enzymes from Bacillus subtilis strain WB600 culture supernatant by anion and cation exchange chromatography and ultrafilatration Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.62 additional information the enzyme shows activity towards complex substrates, e.g. skimmed milk Bacillus subtilis ?
-
 ?
3.4.21.62 succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-methyl-coumaryl-7-amide + H2O
-
 Bacillus subtilis succinyl-L-Ala-L-Ala-L-Pro-L-Phe + 7-amino-4-methylcoumarin
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.62 Subtilisin E
-
 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.62 37
-
 assay at Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.62 8.5
-
 assay at Bacillus subtilis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.4.21.62 700
-
 pH 7.5, 37°C, recombinant wild-type enzyme Bacillus subtilis guanidinium hydrochloride
3.4.21.62 960
-
 pH 7.5, 37°C, recombinant mutant N218S Bacillus subtilis guanidinium hydrochloride
3.4.21.62 1260
-
 pH 7.5, 37°C, recombinant mutant T224A Bacillus subtilis guanidinium hydrochloride
3.4.21.62 2730
-
 pH 7.5, 37°C, recombinant mutant S62I/A153V/G166S/I205V Bacillus subtilis guanidinium hydrochloride
3.4.21.62 3070
-
 pH 7.5, 37°C, recombinant mutant S62I/A153V/G166S/T224A/T240S Bacillus subtilis guanidinium hydrochloride
3.4.21.62 4650
-
 pH 7.5, 37°C, recombinant mutant S62I/A153V/G166S/I205V/N218S/T224A Bacillus subtilis guanidinium hydrochloride