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Literature summary extracted from

  • Marques, J.C.; Lamosa, P.; Russell, C.; Ventura, R.; Maycock, C.; Semmelhack, M.F.; Miller, S.T.; Xavier, K.B.
    Processing the interspecies quorum-sensing signal autoinducer-2 (AI-2): characterization of phospho-(S)-4,5-dihydroxy-2,3-pentanedione isomerization by LsrG protein (2011), J. Biol. Chem., 286, 18331-18343.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.32 overexpression in Escherichia coli as an N-terminal His6-tagged fusion protein Escherichia coli O55:H7

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.3.1.32 crystals of wild-type enzyme with the His6 tag removed are grown via the sitting drop method with a well solution of 1.65 M sodium citrate, pH 6.5. Crystals are cryoprotected in paratone and flash-frozen in the diffractometer cryostream Escherichia coli O55:H7

Protein Variants

EC Number Protein Variants Comment Organism
5.3.1.32 E54A no activity Escherichia coli O55:H7
5.3.1.32 H65A no activity Escherichia coli O55:H7
5.3.1.32 H70A specific activity is about 1% compared to wild-type activity Escherichia coli O55:H7
5.3.1.32 N25A specific activity is about 35% compared to wild-type activity Escherichia coli O55:H7

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.32 diethyldicarbonate
-
 Escherichia coli O55:H7

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.1.32 (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione Escherichia coli O55:H7 (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione i.e. autoinducer 2 i.e. AI-2. The enzyme participates in a degradation pathway of the bacterial quorum-sensing autoinducer molecule AI-2. AI-2 mediates interspecies communication and facilitates regulation of bacterial behaviors such as biofilm formation and virulence 3-hydroxy-5-phosphonooxypentane-2,4-dione
-
 ?
5.3.1.32 (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione Escherichia coli O55:H7 CB9615 (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione i.e. autoinducer 2 i.e. AI-2. The enzyme participates in a degradation pathway of the bacterial quorum-sensing autoinducer molecule AI-2. AI-2 mediates interspecies communication and facilitates regulation of bacterial behaviors such as biofilm formation and virulence 3-hydroxy-5-phosphonooxypentane-2,4-dione
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.32 Escherichia coli O55:H7 D3QT67
-
-
5.3.1.32 Escherichia coli O55:H7 CB9615 D3QT67
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.1.32
-
 Escherichia coli O55:H7

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.3.1.32 0.8
-
 pH 7.2, 30°C, mutant enzyme H70A Escherichia coli O55:H7
5.3.1.32 20.4
-
 pH 7.2, 30°C, mutant enzyme N25A Escherichia coli O55:H7
5.3.1.32 61
-
 pH 7.2, 30°C, wild-type enzyme Escherichia coli O55:H7

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.32 (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione i.e. autoinducer 2 i.e. AI-2. The enzyme participates in a degradation pathway of the bacterial quorum-sensing autoinducer molecule AI-2. AI-2 mediates interspecies communication and facilitates regulation of bacterial behaviors such as biofilm formation and virulence Escherichia coli O55:H7 3-hydroxy-5-phosphonooxypentane-2,4-dione
-
 ?
5.3.1.32 (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione i.e. autoinducer 2 i.e. AI-2 Escherichia coli O55:H7 3-hydroxy-5-phosphonooxypentane-2,4-dione
-
 ?
5.3.1.32 (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione i.e. autoinducer 2 i.e. AI-2. The enzyme participates in a degradation pathway of the bacterial quorum-sensing autoinducer molecule AI-2. AI-2 mediates interspecies communication and facilitates regulation of bacterial behaviors such as biofilm formation and virulence Escherichia coli O55:H7 CB9615 3-hydroxy-5-phosphonooxypentane-2,4-dione
-
 ?
5.3.1.32 (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione i.e. autoinducer 2 i.e. AI-2 Escherichia coli O55:H7 CB9615 3-hydroxy-5-phosphonooxypentane-2,4-dione
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.3.1.32 lsrG
-
 Escherichia coli O55:H7
5.3.1.32 phospho-AI-2 isomerase
-
 Escherichia coli O55:H7

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.3.1.32 30
-
 assay at Escherichia coli O55:H7

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.32 7.2
-
 assay at Escherichia coli O55:H7

General Information

EC Number General Information Comment Organism
5.3.1.32 malfunction an lsrG deletion mutant accumulates at least 10times more (4S)-4-hydroxy-2,3-pentanedione 5-phosphate than wild type cells. The lsrG mutant has increased expression of the lsr operon and an altered profile of (S)-4,5-dihydroxy-2,3-pentanedione (autoinducer-2, AI-2) accumulation and removal Escherichia coli O55:H7
5.3.1.32 metabolism the enzyme participates in a degradation pathway of the bacterial quorum-sensing autoinducer molecule AI-2 Escherichia coli O55:H7