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Literature summary extracted from

  • Hess, V.; Vitt, S.; Müller, V.
    A caffeyl-coenzyme A synthetase initiates caffeate activation prior to caffeate reduction in the acetogenic bacterium Acetobacterium woodii (2011), J. Bacteriol., 193, 971-978.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.12 overexpression in Escherichia coli Acetobacterium woodii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.12 0.013
-
 ATP 30°C, pH 7.0 Acetobacterium woodii
6.2.1.12 0.025
-
 caffeate 30°C, pH 7.0 Acetobacterium woodii
6.2.1.12 0.5
-
 CoA 30°C, pH 7.0 Acetobacterium woodii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.3.23 soluble
-
 Acetobacterium woodii
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.12 K+ strictly K+-dependent. Half-maximal activity is obtained at 6 mM K+ Acetobacterium woodii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.3.23 57700
-
 x * 57700, calculated Acetobacterium woodii
6.2.1.12 60000
-
 x * 60000, SDS-PAGE Acetobacterium woodii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.12 ATP + caffeate + CoA Acetobacterium woodii the enzyme is involved in the reduction of the carbon-carbon double bond of phenylacrylates such as caffeate. The overall reaction is coupled to ATP synthesis by a chemiosmotic mechanism with Na+ as the coupling ion AMP + diphosphate + caffeoyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.8.3.23 Acetobacterium woodii F1CYZ5
-
-
6.2.1.12 Acetobacterium woodii F1CYZ6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.12
-
 Acetobacterium woodii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.12 ATP + 4-coumarate + CoA 52% of the activity with caffeate Acetobacterium woodii AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + caffeate + CoA
-
 Acetobacterium woodii AMP + diphosphate + caffeoyl-CoA
-
 ?
6.2.1.12 ATP + caffeate + CoA the enzyme is involved in the reduction of the carbon-carbon double bond of phenylacrylates such as caffeate. The overall reaction is coupled to ATP synthesis by a chemiosmotic mechanism with Na+ as the coupling ion Acetobacterium woodii AMP + diphosphate + caffeoyl-CoA
-
 ?
6.2.1.12 ATP + cinnamate + CoA 9% of the activity with caffeate Acetobacterium woodii AMP + diphosphate + cinnamoyl-CoA
-
 ?
6.2.1.12 ATP + ferulate + CoA 16% of the activity with caffeate Acetobacterium woodii AMP + diphosphate + feruloyl-CoA
-
 ?
6.2.1.12 additional information no activity with either sinapate or 4-hydroxybenzoate. The more hydroxyl groups are present, the better is the activity of the enzyme. Methoxy groups on the aromatic ring have a negative effect on enzyme activity. It is possible that methoxy groups cause a steric obstruction. No activity can be measured if there is no acryl group present in the substrate Acetobacterium woodii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.3.23 ? x * 57700, calculated Acetobacterium woodii
6.2.1.12 ? x * 60000, SDS-PAGE Acetobacterium woodii

Synonyms

EC Number Synonyms Comment Organism
2.8.3.23 CarA
-
 Acetobacterium woodii
2.8.3.23 YdiF
-
 Acetobacterium woodii
6.2.1.12 caffeoyl-CoA ligase
-
 Acetobacterium woodii
6.2.1.12 caffeoyl-CoA synthetase
-
 Acetobacterium woodii
6.2.1.12 caffeoyl-coenzyme A synthetase
-
 Acetobacterium woodii
6.2.1.12 CarB
-
 Acetobacterium woodii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.2.1.12 25 40
-
 Acetobacterium woodii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.2.1.12 5.5
-
 CoA 30°C, pH 7.0 Acetobacterium woodii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.12 6 8
-
 Acetobacterium woodii

Expression

EC Number Organism Comment Expression
2.8.3.23 Acetobacterium woodii there is no induction by p-hydroxybenzoate or syringate additional information
2.8.3.23 Acetobacterium woodii expression of the car operon is induced by caffeate, p-coumarate, ferulate, and cinnamate and also by sinapate up
6.2.1.12 Acetobacterium woodii CarE and CarD (and thus the caffeate reduction operon) are induced in cells grown in the presence of cinnamate, sinapate, caffeate, ferulate, or 4-coumarate up

General Information

EC Number General Information Comment Organism
2.8.3.23 physiological function coenzyme A transferase CarA, acyl-CoA synthetase CarB, and acyl-CoA dehydrogenase CarC are part of the caffeate respiration pathway Acetobacterium woodii
6.2.1.12 metabolism the enzyme is involved in the reduction of the carbon-carbon double bond of phenylacrylates such as caffeate. The overall reaction is coupled to ATP synthesis by a chemiosmotic mechanism with Na+ as the coupling ion Acetobacterium woodii