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Literature summary extracted from

  • Wang, S.; Yang, Y.; Yang, R.; Zhang, J.; Chen, M.; Matsukawa, S.; Xie, J.; Wei, D.
    Cloning and characterization of a cold-adapted endo-1,5-alpha-L-arabinanase from Paenibacillus polymyxa and rational design for acidic applicability (2014), J. Agric. Food Chem., 62, 8460-8469.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.99 expressed in Escherichia coli BL21(DE3) cells Paenibacillus polymyxa

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.99 H218D the mutant shows higher catalytic efficiency, kcat is improved about 45% versus that of the wild type enzyme Paenibacillus polymyxa
3.2.1.99 H218E the mutant is active, but it does not change the enzymatic property obviously under acidic condition compared with the wild type enzyme Paenibacillus polymyxa
3.2.1.99 H218K inactive Paenibacillus polymyxa
3.2.1.99 H218R inactive Paenibacillus polymyxa
3.2.1.99 H297D inactive Paenibacillus polymyxa
3.2.1.99 H297E inactive Paenibacillus polymyxa
3.2.1.99 H297K inactive Paenibacillus polymyxa
3.2.1.99 H297R inactive Paenibacillus polymyxa
3.2.1.99 H48D inactive Paenibacillus polymyxa
3.2.1.99 H48E inactive Paenibacillus polymyxa
3.2.1.99 H48K inactive Paenibacillus polymyxa
3.2.1.99 H48R inactive Paenibacillus polymyxa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.99 0.1177
-
 linear 1,5-alpha-L-arabinan at 40°C and pH 6.0 Paenibacillus polymyxa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.99 35477
-
 x * 35477, calculated from amino acid sequence Paenibacillus polymyxa

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.99 Paenibacillus polymyxa V5LER1
-
-
3.2.1.99 Paenibacillus polymyxa Z6 V5LER1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.99 Ni-Sepharose column chromatography Paenibacillus polymyxa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.99 73.4
-
 with linear linear 1,5-alpha-L-arabinan as substrate, at 40°C, pH 6.0 Paenibacillus polymyxa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.99 linear 1,5-alpha-L-arabinan + H2O
-
 Paenibacillus polymyxa oligo-alpha-(1->5)-L-arabinoside
-
 ?
3.2.1.99 linear 1,5-alpha-L-arabinan + H2O
-
 Paenibacillus polymyxa Z6 oligo-alpha-(1->5)-L-arabinoside
-
 ?
3.2.1.99 additional information no activity with 4-nitrophenyl-alpha-L-arabinofuranoside, beechwood xylan, wheat flour arabinoxylan, larch arabinogalactan, gum arabic, and soluble starch Paenibacillus polymyxa ?
-
 ?
3.2.1.99 additional information no activity with 4-nitrophenyl-alpha-L-arabinofuranoside, beechwood xylan, wheat flour arabinoxylan, larch arabinogalactan, gum arabic, and soluble starch Paenibacillus polymyxa Z6 ?
-
 ?
3.2.1.99 red debranched arabinan + H2O
-
 Paenibacillus polymyxa oligo-alpha-(1->5)-L-arabinoside
-
 ?
3.2.1.99 red debranched arabinan + H2O
-
 Paenibacillus polymyxa Z6 oligo-alpha-(1->5)-L-arabinoside
-
 ?
3.2.1.99 sugar beet arabinan + H2O
-
 Paenibacillus polymyxa oligo-alpha-(1->5)-L-arabinoside
-
 ?
3.2.1.99 sugar beet arabinan + H2O
-
 Paenibacillus polymyxa Z6 oligo-alpha-(1->5)-L-arabinoside
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.99 ? x * 35477, calculated from amino acid sequence Paenibacillus polymyxa

Synonyms

EC Number Synonyms Comment Organism
3.2.1.99 AbnZ1
-
 Paenibacillus polymyxa
3.2.1.99 endo-1,5-alpha-L-arabinase
-
 Paenibacillus polymyxa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.99 40
-
-
 Paenibacillus polymyxa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.99 5
-
 the specific activity of the enzyme remains 54.1% of maximum at 5°C Paenibacillus polymyxa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.99 68.7
-
 linear 1,5-alpha-L-arabinan wild type enzyme, at 40°C and pH 6.0 Paenibacillus polymyxa
3.2.1.99 99.7
-
 linear 1,5-alpha-L-arabinan mutant enzyme H218D, at 40°C and pH 6.0 Paenibacillus polymyxa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.99 6
-
-
 Paenibacillus polymyxa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.99 5 7 the enzyme achieves about 20.4 and 83.6% of the maximal activity at pH 5.0 and 7.0, respectively Paenibacillus polymyxa

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.99 5 7 the enzyme is stable at pH 7.0 compared with 55.5 and 5.78% of maximal activity remaining after 30 min of incubation at pH 6.0 and 5.0 respectively Paenibacillus polymyxa

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.99 Paenibacillus polymyxa calculated from amino acid sequence
-
 9.2