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Literature summary extracted from

  • Konno, N.; Nakade, K.; Nishitani, Y.; Mizuno, M.; Sakamoto, Y.
    Lentinan degradation in the Lentinula edodes fruiting body during postharvest preservation is reduced by downregulation of the exo-beta-1,3-glucanase EXG2 (2014), J. Agric. Food Chem., 62, 8153-8157.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.58 gene exg2, recombinant overexpression in Lentinula edodes strain SR-1 Lentinula edodes

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.58 additional information downregulation of the expression of an exo-beta-1,3-glucanase, exg2, using RNA interference, phenotype, overview Lentinula edodes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.58 lentinan + H2O Lentinula edodes lentinan content in fruiting bodies decreases after harvesting and storage due to an increase in glucanase activity ?
-
 ?
3.2.1.58 lentinan + H2O Lentinula edodes SR-1 lentinan content in fruiting bodies decreases after harvesting and storage due to an increase in glucanase activity ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.58 Lentinula edodes
-
 gene exg2
-
3.2.1.58 Lentinula edodes SR-1
-
 gene exg2
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.58 fruit body
-
 Lentinula edodes
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.58 4-nitrophenyl-N-acetyl-beta-D-glucosaminide + H2O
-
 Lentinula edodes 4-nitrophenol + N-acetyl-beta-D-glucosaminide
-
 ?
3.2.1.58 curdlan + H2O
-
 Lentinula edodes ?
-
 ?
3.2.1.58 curdlan + H2O
-
 Lentinula edodes SR-1 ?
-
 ?
3.2.1.58 lentinan + H2O
-
 Lentinula edodes ?
-
 ?
3.2.1.58 lentinan + H2O lentinan content in fruiting bodies decreases after harvesting and storage due to an increase in glucanase activity Lentinula edodes ?
-
 ?
3.2.1.58 lentinan + H2O
-
 Lentinula edodes SR-1 ?
-
 ?
3.2.1.58 lentinan + H2O lentinan content in fruiting bodies decreases after harvesting and storage due to an increase in glucanase activity Lentinula edodes SR-1 ?
-
 ?
3.2.1.58 additional information enzyme activity with different substrates in wild-type and mutant strains, overview Lentinula edodes ?
-
 ?
3.2.1.58 additional information enzyme activity with different substrates in wild-type and mutant strains, overview Lentinula edodes SR-1 ?
-
 ?
3.2.1.58 pustulan + H2O
-
 Lentinula edodes ?
-
 ?
3.2.1.58 pustulan + H2O
-
 Lentinula edodes SR-1 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.58 EXG2
-
 Lentinula edodes
3.2.1.58 exo-beta-1,3-glucanase
-
 Lentinula edodes

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.58 37
-
 assay at Lentinula edodes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.58 4.2
-
 assay at Lentinula edodes

General Information

EC Number General Information Comment Organism
3.2.1.58 malfunction the enzyme-downregulated strain shows significantly lower lentinan degrading activity (60-70% of the wild-type strain) in the fruiting bodies 2-4 days after harvesting. The lentinan content of fresh fruiting bodies is similar in the wild-type and EXG2 downregulated strains, but in the downregulated strain, only 25.4% of the lentinan is lost after 4 days, indicating that downregulation of EXG2 enables keeping the lentinan content high longer Lentinula edodes