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Literature summary extracted from

  • Zhou, Y.; Dong, F.; Kunimasa, A.; Zhang, Y.; Cheng, S.; Lu, J.; Zhang, L.; Murata, A.; Mayer, F.; Fleischmann, P.; Watanabe, N.; Yang, Z.
    Occurrence of glycosidically conjugated 1-phenylethanol and its hydrolase beta-primeverosidase in tea (Camellia sinensis) flowers (2014), J. Agric. Food Chem., 62, 8042-8050.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.149 cloned from tea flowers, recombinant inducible expression of His-tagged enzyme in Escherichia coli Camellia sinensis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.149 cell wall and in the cavity area among cells Camellia sinensis 5618
-

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.149 Camellia sinensis Q7X9A9 var. Jinxuan
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.149 recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography and desalting gel filtration Camellia sinensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.149 anther low expression level Camellia sinensis
-
3.2.1.149 flower
-
 Camellia sinensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.149 (R)-1-phenylethyl beta-D-glucopyranoside + H2O LC-MS analysis and quantitative GC-MS analysis of substrate and product Camellia sinensis (R)-1-phenylethanol + beta-D-glucopyranose
-
 ?
3.2.1.149 (R)-1-phenylethyl beta-primeveroside + H2O LC-MS analysis and quantitative GC-MS analysis of substrate and product Camellia sinensis (R)-1-phenylethanol + beta-primeverose
-
 ?
3.2.1.149 (S)-1-phenylethyl beta-D-glucopyranoside + H2O LC-MS analysis and quantitative GC-MS analysis of substrate and product Camellia sinensis (S)-1-phenylethanol + beta-D-glucopyranose
-
 ?
3.2.1.149 (S)-1-phenylethyl beta-primeveroside + H2O LC-MS analysis and quantitative GC-MS analysis of substrate and product Camellia sinensis (S)-1-phenylethanol + beta-primeverose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.149 37
-
 assay at Camellia sinensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.149 5
-
 assay at Camellia sinensis