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Literature summary extracted from

  • Watanabe, S.; Kakudo, A.; Ohta, M.; Mita, K.; Fujiyama, K.; Inumaru, S.
    Molecular cloning and characterization of the alpha-glucosidase II from Bombyx mori and Spodoptera frugiperda (2013), Insect Biochem. Mol. Biol., 43, 319-327.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.207 recombinant expression of His-tagged alpha-subunits via the vaculovirus transfection system in Sf21AE cells Spodoptera frugiperda
3.2.1.207 recombinant expression of His-tagged and GFP-tagged alpha-subunit via the vaculovirus transfection system in Sf21AE cells Bombyx mori
3.2.1.207 recombinant expression of His-tagged and GFP-tagged beta-subunits via the vaculovirus transfection system in Sf21AE cells Bombyx mori

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.207 1,4-dideoxy-1,4-imino-D-arabinitol
-
Bombyx mori
3.2.1.207 1,4-dideoxy-1,4-imino-D-arabinitol
-
Spodoptera frugiperda
3.2.1.207 1-deoxynojirimycin
-
Bombyx mori
3.2.1.207 1-deoxynojirimycin
-
Spodoptera frugiperda
3.2.1.207 castanospermine
-
Bombyx mori
3.2.1.207 castanospermine
-
Spodoptera frugiperda
3.2.1.207 additional information the enzyme from silkworm is not sensitive to the alpha-glucosidase inhibitors from mulberry leaves; the enzyme from silkworm is not very sensitive to the alpha-glucosidase inhibitors from mulberry leaves Bombyx mori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.207 additional information
-
additional information Michaelis-Menten kinetics Bombyx mori
3.2.1.207 additional information
-
additional information Michaelis-Menten kinetics Spodoptera frugiperda
3.2.1.207 2.37
-
4-nitrophenyl alpha-D-glucopyranoside recombinant enzyme, pH 6,5, 37°C Spodoptera frugiperda
3.2.1.207 2.82
-
4-nitrophenyl alpha-D-glucopyranoside recombinant enzyme, pH 6,5, 37°C Bombyx mori

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.207 endoplasmic reticulum
-
Bombyx mori 5783
-
3.2.1.207 endoplasmic reticulum
-
Spodoptera frugiperda 5783
-
3.2.1.207 additional information the enzyme has a signal peptide in the N-terminus Bombyx mori
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.207 105000
-
alphabeta, x * 105000, recombinant alpha-subunit, SDS-PAGE, beta-subunit not specified in the publication Bombyx mori
3.2.1.207 105206
-
alphabeta, x * 105206, alpha-subunit, sequence calculation, x * 106000, recombinant alpha-subunit, SDS-PAGE, beta-subunit not specified in the publication Spodoptera frugiperda
3.2.1.207 106000
-
alphabeta, x * 105206, alpha-subunit, sequence calculation, x * 106000, recombinant alpha-subunit, SDS-PAGE, beta-subunit not specified in the publication Spodoptera frugiperda

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.207 Bombyx mori L8B6C5 beta-subunit
-
3.2.1.207 Bombyx mori L8B8U1 alpha-subunit
-
3.2.1.207 Spodoptera frugiperda L8B8V1 alpha-subunit
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.207 recombinant His-tagged and GFP-tagged subunit by nickel affinity chromatography Bombyx mori

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.207 BmN cell
-
Bombyx mori
-
3.2.1.207 Sf21AE cell
-
Spodoptera frugiperda
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.207 4.53
-
recombinant enzyme, pH 6,5, 37°C, substrate 4-nitrophenyl alpha-D-glucopyranoside Bombyx mori
3.2.1.207 4.65
-
recombinant enzyme, pH 6,5, 37°C, substrate 4-nitrophenyl alpha-D-glucopyranoside Spodoptera frugiperda

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.207 4-nitrophenyl alpha-D-glucopyranoside + H2O
-
Spodoptera frugiperda 4-nitrophenol + alpha-D-glucopyranose
-
?
3.2.1.207 4-nitrophenyl alpha-D-glucopyranoside + H2O cleavage by the single alpha-subunit, the beta-subunit is not required for activity Bombyx mori 4-nitrophenol + alpha-D-glucopyranose
-
?
3.2.1.207 additional information no activity of the single alpha-subunit with N-glycan, the enzyme complex of the alpha- and beta-subunits is required for N-glycan cleavage Bombyx mori ?
-
?
3.2.1.207 additional information no activity with N-glycan Spodoptera frugiperda ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.207 heterodimer alphabeta Bombyx mori
3.2.1.207 heterodimer alphabeta, x * 105000, recombinant alpha-subunit, SDS-PAGE, beta-subunit not specified in the publication Bombyx mori
3.2.1.207 heterodimer alphabeta, x * 105206, alpha-subunit, sequence calculation, x * 106000, recombinant alpha-subunit, SDS-PAGE, beta-subunit not specified in the publication Spodoptera frugiperda

Synonyms

EC Number Synonyms Comment Organism
3.2.1.207 alpha-glucosidase II
-
Bombyx mori
3.2.1.207 alpha-glucosidase II
-
Spodoptera frugiperda

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.207 37
-
assay at Bombyx mori

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.207 6.5
-
assay at Bombyx mori

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.2.1.207 0.0079
-
recombinant enzyme, pH 6,5, 37°C Spodoptera frugiperda 1-deoxynojirimycin
3.2.1.207 0.0195
-
recombinant enzyme, pH 6,5, 37°C Spodoptera frugiperda castanospermine
3.2.1.207 0.0501
-
recombinant enzyme, pH 6,5, 37°C Bombyx mori castanospermine
3.2.1.207 0.0708
-
recombinant enzyme, pH 6,5, 37°C Spodoptera frugiperda 1,4-dideoxy-1,4-imino-D-arabinitol
3.2.1.207 0.0727
-
recombinant enzyme, pH 6,5, 37°C Bombyx mori 1-deoxynojirimycin
3.2.1.207 0.5102
-
recombinant enzyme, pH 6,5, 37°C Bombyx mori 1,4-dideoxy-1,4-imino-D-arabinitol

General Information

EC Number General Information Comment Organism
3.2.1.207 evolution the enzyme contains a glycoside hydrolase family 31 motif containing the conserved WXDMNE sequence, which is essential for enzymatic activity Bombyx mori
3.2.1.207 additional information high concentration of alpha-glucosidase inhibitors from mulberry leaves accumulate in Bombyx mori by feeding, silkworm does not show any toxic symptom against these inhibitors and N-glycosylation of recombinant proteins is not affected Bombyx mori
3.2.1.207 physiological function the enzyme is important for N-glycosylation processing and quality control of nascent glycoproteins Bombyx mori
3.2.1.207 physiological function the enzyme is important for N-glycosylation processing and quality control of nascent glycoproteins Spodoptera frugiperda