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Literature summary extracted from

  • Ramos-Parra, P.; Urrea-Lopez, R.; Diaz de la Garza, R.
    Folate analysis in complex food matrices: Use of a recombinant Arabidopsis ?-glutamyl hydrolase for folate deglutamylation (2013), Food Res. Int., 54, 177-185.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.19.9 analysis folate analysis. Isoform GGH2 is able to completely deconjugate folates using 50-100 microg/g of sample for 1 h at 37°C in all matrices tested. It removes the tails of folylpolyglutamates from tomato fruit, black bean and peanut seeds, and alfalfa sprouts extracts Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.19.9 expression in Escherichia coli Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
3.4.19.9 Arabidopsis thaliana O65355 isoform GGH2
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.19.9 5,10-methenyltetrahydrofolate glutamate + H2O
-
 Arabidopsis thaliana 5,10-methenyltetrahydrofolate + L-glutamate
-
 ?
3.4.19.9 5,10-methenyltetrahydrofolate heptaglutamate + 7 H2O
-
 Arabidopsis thaliana 5,10-methenyltetrahydrofolate + 7 L-glutamate
-
 ?
3.4.19.9 5,10-methenyltetrahydrofolate hexaglutamate + 6 H2O
-
 Arabidopsis thaliana 5,10-methenyltetrahydrofolate + 6 L-glutamate
-
 ?
3.4.19.9 5-formyltetrahydrofolate glutamate + H2O
-
 Arabidopsis thaliana 5-formyltetrahydrofolate + L-glutamate
-
 ?
3.4.19.9 5-formyltetrahydrofolate heptaglutamate + 7 H2O
-
 Arabidopsis thaliana 5-formyltetrahydrofolate + 7 L-glutamate
-
 ?
3.4.19.9 5-formyltetrahydrofolate hexaglutamate + 6 H2O
-
 Arabidopsis thaliana 5-formyltetrahydrofolate + 6 L-glutamate
-
 ?
3.4.19.9 5-methyltetrahydrofolate glutamate + H2O
-
 Arabidopsis thaliana 5-methyltetrahydrofolate + L-glutamate
-
 ?
3.4.19.9 5-methyltetrahydrofolate heptaglutamate + 7 H2O
-
 Arabidopsis thaliana 5-methyltetrahydrofolate + 7 L-glutamate
-
 ?
3.4.19.9 5-methyltetrahydrofolate hexaglutamate + 6 H2O
-
 Arabidopsis thaliana 5-methyltetrahydrofolate + 6 L-glutamate
-
 ?
3.4.19.9 additional information isoform GGH2 alone is able to completely deconjugate folates using 50-100 microg/g of sample for 1 h at 37°C in all matrices tested. It removes the tails of folylpolyglutamates from tomato fruit, black bean and peanut seeds, and alfalfa sprouts extracts Arabidopsis thaliana ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.19.9 At1g78680
-
 Arabidopsis thaliana
3.4.19.9 GGH2
-
 Arabidopsis thaliana