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Literature summary extracted from

  • Navarrete-del-Toro, M.A.; Garcia-Carreno, F.L.; Hernandez-Cortes, P.; Molnar, T.; Graf, L.
    Biochemical characterisation of chymotrypsin from the midgut gland of yellowleg shrimp, Penaeus californiensis (2015), Food Chem., 173, 147-155.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.1 phenylmethylsulfonyl fluoride complete inhibition Penaeus californiensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.1 0.12
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide pH 8.0, 25°C Penaeus californiensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.1 23720
-
 x * 27000, SDS-PAGE, x * 23720, calculated Penaeus californiensis
3.4.21.1 27000
-
 x * 27000, SDS-PAGE, x * 23720, calculated Penaeus californiensis

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.1 Penaeus californiensis U5KGX4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.1
-
 Penaeus californiensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.1 digestive gland
-
 Penaeus californiensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.1 Bovine serum albumin + H2O
-
 Penaeus californiensis ? partial hydrolysis ?
3.4.21.1 native collagen + H2O
-
 Penaeus californiensis ?
-
 ?
3.4.21.1 succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide + H2O
-
 Penaeus californiensis succinyl-L-Ala-L-Ala-L-Pro-L-Phe + 4-nitroaniline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.1 ? x * 27000, SDS-PAGE, x * 23720, calculated Penaeus californiensis

Synonyms

EC Number Synonyms Comment Organism
3.4.21.1 CHY20
-
 Penaeus californiensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.1 30
-
 120 min, 20-40% loss of activity Penaeus californiensis
3.4.21.1 50
-
 30 min, complete loss of activity Penaeus californiensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.1 21.5
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide pH 8.0, 25°C Penaeus californiensis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.21.1 4
-
 120 min, 75% residual activity Penaeus californiensis
3.4.21.1 5 10 120 min, no loss of activity Penaeus californiensis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.21.1 Penaeus californiensis isoelectric focusing
-
 3.6

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.21.1 179
-
 succinyl-L-Ala-L-Ala-L-Pro-L-Phe-4-nitroanilide pH 8.0, 25°C Penaeus californiensis