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BRENDA support

Literature summary extracted from

  • Ilaria, B.; Marco, E.; Katia, L.; Maria Vittoria, G.A.
    Pineapple stem bromelain immobilized on different supports: catalytic properties in model wine (2012), Biotechnol. Prog., 28, 1472-1477.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.22.32 food industry stem bromelain immobilized on chitosan beads without glutaraldehyde yields a food-safe biocatalyst for unstable real wine future application Ananas comosus

General Stability

EC Number General Stability Organism
3.4.22.32 immobilized bromelain shows a higher half-life respect to the free enzyme. The addition of free cysteine during immobilization phase, improves bromelain half-life more than 4folds Ananas comosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.32 0.2497
-
 Bz-Phe-Val-Arg-4-nitroanilide free enzyme, at pH 3.2 and 25°C Ananas comosus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.32 Ananas comosus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.32 stem
-
 Ananas comosus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.32 Bz-Phe-Val-Arg-4-nitroanilide + H2O
-
 Ananas comosus Bz-Phe-Val-Arg + 4-nitroaniline
-
 ?
3.4.22.32 Z-Arg-Arg-4-nitroanilide + H2O
-
 Ananas comosus ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.32 20.03
-
 Bz-Phe-Val-Arg-4-nitroanilide free enzyme, at pH 3.2 and 25°C Ananas comosus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.22.32 3.2 7 stem bromelain solubilized at pH 7.0 and at pH 3.2 retains, after 16 h at 256°C, 10 and 40% of the initial activity, respectively Ananas comosus