Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chen, X.; Zhai, C.; Kang, L.; Li, C.; Yan, H.; Zhou, Y.; Yu, X.; Ma, L.
    High-level expression and characterization of a highly thermostable chitosanase from Aspergillus fumigatus in Pichia pastoris (2012), Biotechnol. Lett., 34, 689-694.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.132 expressed in Pichia pastoris GS115 cells Aspergillus fumigatus

General Stability

EC Number General Stability Organism
3.2.1.132 the enzyme retaines 70% activity after incubation with 10 M urea at room temperature for 30 min Aspergillus fumigatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.132 Ag+ 10 mM inhibits more than 50% of the enzyme activity Aspergillus fumigatus
3.2.1.132 Cu2+ 10 mM inhibits more than 50% of the enzyme activity Aspergillus fumigatus
3.2.1.132 Fe3+ strongly inhibited by 10 mM Aspergillus fumigatus
3.2.1.132 SDS strongly inhibited by 0.1% (w/v) SDS Aspergillus fumigatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.132 28000
-
 x * 28000, SDS-PAGE Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.132 chitosan + H2O Aspergillus fumigatus
-
 chitosan oligosaccharides
-
 ?
3.2.1.132 chitosan + H2O Aspergillus fumigatus Y2K
-
 chitosan oligosaccharides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.132 Aspergillus fumigatus Q875I9 synonym Aspergillus fumigatus
-
3.2.1.132 Aspergillus fumigatus Y2K Q875I9 synonym Aspergillus fumigatus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.132 chitosan + H2O
-
 Aspergillus fumigatus chitosan oligosaccharides
-
 ?
3.2.1.132 chitosan + H2O
-
 Aspergillus fumigatus Y2K chitosan oligosaccharides
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.132 ? x * 28000, SDS-PAGE Aspergillus fumigatus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.132 Csn
-
 Aspergillus fumigatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.132 55 65
-
 Aspergillus fumigatus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.132 45 75 about 50% activity at 45°C, about 80% activity at 50°C, 100% activity from 55-65°C, about 80% activity at 70°C, about 50% activity at 75°C Aspergillus fumigatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.132 80 100 the enzyme has half-lives of 2.5 h at 80°C, 1 h at 90°C and 32 min at 100°C. Almost full enzyme activity remains after 1 h at 80°C, 30 min at 90°C and 10 min at 100°C Aspergillus fumigatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.132 6.5
-
-
 Aspergillus fumigatus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.132 4 7.5 more than 50% activity between pH 4.0 and 7.5, more than 90% activity between pH 4.5 and 7.0 Aspergillus fumigatus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.132 3.5 8 the enzyme retains 100% activity after 1 h in the pH range 3.5-8.0 Aspergillus fumigatus