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Literature summary extracted from

  • Wu, I.; Arnold, F.H.
    Engineered thermostable fungal Cel6A and Cel7A cellobiohydrolases hydrolyze cellulose efficiently at elevated temperatures (2013), Biotechnol. Bioeng., 110, 1874-1883.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.91 expressed in Saccharomyces cerevisiae strain YDR483W BY4742 Humicola insolens
3.2.1.91 expressed in Saccharomyces cerevisiae strain YDR483W BY4742 Trichoderma reesei
3.2.1.91 expressed in Saccharomyces cerevisiae strain YDR483W BY4742 Thermochaetoides thermophila

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.91 A385P the mutant shows a T50 value of 64.7°C Humicola insolens
3.2.1.91 M134L the mutant shows a T50 value of 62°C Trichoderma reesei
3.2.1.91 M235P the mutant shows a T50 value of 63.1°C Humicola insolens
3.2.1.91 Q276L the mutant shows a T50 value of 58.6°C Trichoderma reesei
3.2.1.91 Q390P the mutant shows a T50 value of 66.1°C Humicola insolens
3.2.1.91 S215P the mutant shows a T50 value of 65.6°C Humicola insolens
3.2.1.91 S30F the mutant shows a T50 value of 60.3°C Trichoderma reesei
3.2.1.91 S316P the mutant shows a T50 value of 61.9°C Trichoderma reesei
3.2.1.91 S406P the mutant shows a T50 value of 61.5°C Trichoderma reesei
3.2.1.91 S413P the mutant shows a T50 value of 63.8°C Trichoderma reesei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.91 cellulose + H2O Humicola insolens
-
 cellobiose + ?
-
 ?
3.2.1.91 cellulose + H2O Trichoderma reesei
-
 cellobiose + ?
-
 ?
3.2.1.91 cellulose + H2O Thermochaetoides thermophila
-
 cellobiose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.91 Humicola insolens
-
-
-
3.2.1.91 Thermochaetoides thermophila
-
-
-
3.2.1.91 Trichoderma reesei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.91 Ni-NTA column chromatography Humicola insolens
3.2.1.91 Ni-NTA column chromatography Trichoderma reesei
3.2.1.91 Ni-NTA column chromatography Thermochaetoides thermophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.91 avicel + H2O
-
 Humicola insolens cellobiose + ?
-
 ?
3.2.1.91 avicel + H2O
-
 Trichoderma reesei cellobiose + ?
-
 ?
3.2.1.91 avicel + H2O
-
 Thermochaetoides thermophila cellobiose + ?
-
 ?
3.2.1.91 cellulose + H2O
-
 Humicola insolens cellobiose + ?
-
 ?
3.2.1.91 cellulose + H2O
-
 Trichoderma reesei cellobiose + ?
-
 ?
3.2.1.91 cellulose + H2O
-
 Thermochaetoides thermophila cellobiose + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.91 Cel6A
-
 Humicola insolens
3.2.1.91 Cel6A
-
 Trichoderma reesei
3.2.1.91 Cel6A
-
 Thermochaetoides thermophila
3.2.1.91 cellobiohydrolase
-
 Humicola insolens
3.2.1.91 cellobiohydrolase
-
 Trichoderma reesei
3.2.1.91 cellobiohydrolase
-
 Thermochaetoides thermophila

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.91 75
-
 the wild type enzyme is inactive at 75°C Thermochaetoides thermophila
3.2.1.91 75
-
 the wild type enzyme shows a half-life of more than 2.5 min at 75°C and T50 of 60.2°C Trichoderma reesei
3.2.1.91 75
-
 the wild type enzyme shows a half-life of more than 2.5 min at 75°C and T50 of 65.2°C Humicola insolens