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Literature summary extracted from

  • Yadav, S.; Anand, G.; Dubey, A.; Yadav, D.
    Purification and characterization of an exo-polygalacturonase secreted by Rhizopus oryzae MTCC 1987 and its role in retting of Crotalaria juncea fibre (2012), Biologia, 67, 1069-1074.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.67 Ag+ complete inhibition at 1 mM Rhizopus arrhizus
3.2.1.67 Ca2+
-
 Rhizopus arrhizus
3.2.1.67 EDTA
-
 Rhizopus arrhizus
3.2.1.67 Hg2+ complete inhibition at 1 mM Rhizopus arrhizus
3.2.1.67 KMnO4 complete inhibition at 1 mM Rhizopus arrhizus
3.2.1.67 additional information poor inhibitory effects by K+ and Mg2+ Rhizopus arrhizus
3.2.1.67 NaN3
-
 Rhizopus arrhizus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.67 additional information
-
 additional information 2.7 mg/ml with citrus polygalacturonic acid, pH 5.0, 50°C Rhizopus arrhizus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.67 extracellular the enzyme is secreted Rhizopus arrhizus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.67 Co2+ activates Rhizopus arrhizus
3.2.1.67 Mn2+ activates Rhizopus arrhizus
3.2.1.67 additional information poor activation by Na+ and potassium ferrocyanide Rhizopus arrhizus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.67 75500
-
 x * 75500, SDS-PAGE Rhizopus arrhizus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.67 Rhizopus arrhizus
-
-
-
3.2.1.67 Rhizopus arrhizus MTCC 1987
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.67 native extracelluar enzyme 16.3fold by ammonium sulfate fractionation and anion exchange chromatography to homogeneity Rhizopus arrhizus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.67 additional information submerged fermentation Rhizopus arrhizus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.67 60.5
-
 with citrus polygalacturonic acid, pH 5.0, 50°C Rhizopus arrhizus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.67 [(1->4)-alpha-D-galacturonide]n + H2O citrus polygalacturonic acid Rhizopus arrhizus [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate
-
 ?
3.2.1.67 [(1->4)-alpha-D-galacturonide]n + H2O citrus polygalacturonic acid Rhizopus arrhizus MTCC 1987 [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.67 ? x * 75500, SDS-PAGE Rhizopus arrhizus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.67 exo-polygalacturonase
-
 Rhizopus arrhizus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.67 50
-
-
 Rhizopus arrhizus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.67 30
-
 purified enzyme, 2 h, completely stable at Rhizopus arrhizus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.67 2230
-
 [(1->4)-alpha-D-galacturonide]n pH 5.0, 50°C Rhizopus arrhizus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.67 5
-
-
 Rhizopus arrhizus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.67 5 12 purified enzyme, 24 h, almost completely stable at Rhizopus arrhizus

General Information

EC Number General Information Comment Organism
3.2.1.67 physiological function the enzyme plays a role in retting of Crotalaria juncea fibre Rhizopus arrhizus