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Literature summary extracted from
- Isolation and characterization of Xenopus soluble epoxide hydrolase (2014), Biochim. Biophys. Acta, 1841, 954-962.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.3.2.10 | expressed in Escherichia coli BL21-CodonPlus (DE3) cells | Xenopus laevis |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.3.2.10 | N29T/H146R | the mutant shows strongly reduced hydrolase activity and does not have phosphatase activity | Xenopus laevis |
| 3.3.2.10 | N29T/H146R/G11D | the mutant shows strongly reduced hydrolase activity and does not have phosphatase activity | Xenopus laevis |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.3.2.10 | 0.01916 | - |
3-phenyl-cyano(6-methoxy-2-naphthalenyl)methyl ester 2-oxiraneacetic acid | at pH 7.0 and 30°C | Xenopus laevis |  |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.3.2.10 | soluble | - |
Xenopus laevis | - |
- |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.3.2.10 | Xenopus laevis | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.3.2.10 | Ni-NTA agarose column chromatography | Xenopus laevis |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.3.2.10 | brain | lowest expression | Xenopus laevis | - |
| 3.3.2.10 | embryo | - |
Xenopus laevis | - |
| 3.3.2.10 | eye | - |
Xenopus laevis | - |
| 3.3.2.10 | heart | - |
Xenopus laevis | - |
| 3.3.2.10 | kidney | - |
Xenopus laevis | - |
| 3.3.2.10 | liver | highest expression | Xenopus laevis | - |
| 3.3.2.10 | lung | - |
Xenopus laevis | - |
| 3.3.2.10 | pancreas | - |
Xenopus laevis | - |
| 3.3.2.10 | spleen | - |
Xenopus laevis | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.3.2.10 | 11,12-epoxyeicosatrienoic acid + H2O | - |
Xenopus laevis | 11,12-dihydroxyeicosatrienoic acid | - |
? | |
| 3.3.2.10 | 14,15-epoxyeicosatrienoic acid + H2O | - |
Xenopus laevis | 14,15-dihydroxyeicosatrienoic acid | - |
? | |
| 3.3.2.10 | 3-phenyl-cyano(6-methoxy-2-naphthalenyl)methyl ester 2-oxiraneacetic acid + H2O | - |
Xenopus laevis | 6-methoxy-2-naphthaldehyde + ? | - |
? | |
| 3.3.2.10 | additional information | the enzyme does not show phosphatase activity toward 4-methylumbelliferyl phosphate or several lysophosphatidic acids | Xenopus laevis | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.3.2.10 | SEH | - |
Xenopus laevis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.3.2.10 | 30 | - |
- |
Xenopus laevis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.3.2.10 | 7 | 7.5 | - |
Xenopus laevis |
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Release 2023.1 (January 2023)
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