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Literature summary extracted from
- Role of cysteine residues in thermal inactivation of fungal Cel6A cellobiohydrolases (2013), Biochim. Biophys. Acta, 1834, 1539-1544.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.91 | expressed in Saccharomyces cerevisiae strain YDR483W BY4742 | Humicola insolens |
| 3.2.1.91 | expressed in Saccharomyces cerevisiae strain YDR483W BY4742 | Trichoderma reesei |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.91 | cellulose + H2O | Humicola insolens | - |
cellobiose + ? | - |
? |  |
| 3.2.1.91 | cellulose + H2O | Trichoderma reesei | - |
cellobiose + ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.91 | Humicola insolens | - |
- |
- |
| 3.2.1.91 | Trichoderma reesei | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.91 | His-Trap column chromatography | Humicola insolens |
| 3.2.1.91 | His-Trap column chromatography | Trichoderma reesei |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.91 | avicel + H2O | - |
Humicola insolens | ? | - |
? | |
| 3.2.1.91 | avicel + H2O | - |
Trichoderma reesei | ? | - |
? | |
| 3.2.1.91 | cellulose + H2O | - |
Humicola insolens | cellobiose + ? | - |
? | |
| 3.2.1.91 | cellulose + H2O | - |
Trichoderma reesei | cellobiose + ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.91 | Cel6A | - |
Humicola insolens |
| 3.2.1.91 | Cel6A | - |
Trichoderma reesei |
| 3.2.1.91 | cellobiohydrolase | - |
Humicola insolens |
| 3.2.1.91 | cellobiohydrolase | - |
Trichoderma reesei |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.91 | 90 | - |
the enzyme is inactivated after 15-minute incubation at 90°C and pH 7.0 | Humicola insolens |
| 3.2.1.91 | 90 | - |
the enzyme is inactivated after 15-minute incubation at 90°C and pH 7.0 | Trichoderma reesei |
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Release 2023.1 (January 2023)
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