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Literature summary extracted from

  • Lin, N.; Ai, T.B.; Gao, J.H.; Fan, L.H.; Wang, S.H.; Chen, F.
    Identification of novel acyl-ACP thioesterase gene ClFATB1 from Cinnamomum longepaniculatum (2013), Biochemistry, 78, 1298-1303.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.2.14 recombinantly expressed in Escherichia coli Cinnamomum longipaniculatum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.2.14 61000
-
 SDS-PAGE, recombinant protein Cinnamomum longipaniculatum

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.14 Cinnamomum longipaniculatum
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.2.14 leaf
-
 Cinnamomum longipaniculatum
-
3.1.2.14 root
-
 Cinnamomum longipaniculatum
-
3.1.2.14 seed
-
 Cinnamomum longipaniculatum
-
3.1.2.14 stem
-
 Cinnamomum longipaniculatum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.14 additional information recombinant ClFATB1 in Escherichia coli has higher specific activities against saturated 16:0- and 18:0-ACPs than on unsaturated 18:1-ACP Cinnamomum longipaniculatum ?
-
 ?
3.1.2.14 oleoyl-[acyl-carrier protein] + H2O
-
 Cinnamomum longipaniculatum oleic acid + acyl-carrier protein
-
 ?
3.1.2.14 palmitoleoyl-[acyl-carrier protein] + H2O
-
 Cinnamomum longipaniculatum palmitoleic acid + acyl-carrier protein
-
 ?
3.1.2.14 stearoyl-ACP + H2O
-
 Cinnamomum longipaniculatum stearate + acyl-carrier protein
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.2.14 ClFATB1
-
 Cinnamomum longipaniculatum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.2.14 25
-
 assay at Cinnamomum longipaniculatum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.2.14 8
-
 assay at Cinnamomum longipaniculatum

General Information

EC Number General Information Comment Organism
3.1.2.14 malfunction overexpression of ClFATB1 in transgenic tobaccos upregulates thioesterase activities of crude proteins against 16:0-ACP and 18:0-ACP by 20.3 and 5.7%, respectively, and results in an increase in the contents of palmitic and stearic acids by 15.4 and 10.5%, respectively. Ectopic expression of this gene decreases the substrate specificities of crude proteins to unsaturated 18:1-ACP by 12.7% in transgenic tobacco and lowers the contents of oleic, linoleic, and linolenic acids in transgenic leaves Cinnamomum longipaniculatum