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Literature summary extracted from

  • Lyu, Q.; Wang, S.; Xu, W.; Han, B.; Liu, W.; Jones, D.N.; Liu, W.
    Structural insights into the substrate-binding mechanism for a novel chitosanase (2014), Biochem. J., 461, 335-345.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.132 expressed in Escherichia coli BL21(DE3) cells Microbacterium sp.

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.132 in complex with hexaglucosamine, hanging drop vapor diffusion method, using 0.05 M KH2PO4 and 23% (w/v) PEG 8000 using 0.7 M NaCl as the reservoir at 15°C Microbacterium sp.

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.132 D235A the mutant shows about 18% of wild type activity Microbacterium sp.
3.2.1.132 D43A inactive Microbacterium sp.
3.2.1.132 E25A inactive Microbacterium sp.
3.2.1.132 H203A the mutant shows about 3% of wild type activity Microbacterium sp.
3.2.1.132 S27A the mutant shows about 80% of wild type activity Microbacterium sp.
3.2.1.132 T58A the mutant shows about 20% of wild type activity Microbacterium sp.
3.2.1.132 Y37F the mutant shows about 5% of wild type activity Microbacterium sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.132 chitosan + H2O Microbacterium sp.
-
 chitosan oligomers chitodimer and chitotrimer as main products, a monoacetyl chitodimer and a monoacetyl chitotrimer but no significant amount of monomer glucosamine or chitotetramer is identified ?
3.2.1.132 chitosan + H2O Microbacterium sp. OU01
-
 chitosan oligomers chitodimer and chitotrimer as main products, a monoacetyl chitodimer and a monoacetyl chitotrimer but no significant amount of monomer glucosamine or chitotetramer is identified ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.132 Microbacterium sp. A7KBW5
-
-
3.2.1.132 Microbacterium sp. OU01 A7KBW5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.132 glutathione-Sepharose 4B bead chromatography, and Sephacryl S-100 gel filtration Microbacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.132 chitohexaose + H2O
-
 Microbacterium sp. ?
-
 ?
3.2.1.132 chitohexaose + H2O
-
 Microbacterium sp. OU01 ?
-
 ?
3.2.1.132 chitosan + H2O
-
 Microbacterium sp. chitosan oligomers chitodimer and chitotrimer as main products, a monoacetyl chitodimer and a monoacetyl chitotrimer but no significant amount of monomer glucosamine or chitotetramer is identified ?
3.2.1.132 chitosan + H2O
-
 Microbacterium sp. OU01 chitosan oligomers chitodimer and chitotrimer as main products, a monoacetyl chitodimer and a monoacetyl chitotrimer but no significant amount of monomer glucosamine or chitotetramer is identified ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.132 42.5
-
 melting temperature of the wild type enzyme Microbacterium sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.132 205
-
 chitosan at pH 5.6 and 37°C Microbacterium sp.