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Literature summary extracted from

  • Lee, Y.S.; Park, D.J.; Choi, Y.L.
    Characterization of maltotriose production by hydrolyzing of soluble starch with alpha-amylase from Microbulbifer thermotolerans DAU221 (2015), Appl. Microbiol. Biotechnol., 99, 3901-3911.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.116 expressed in Escherichia coli BL21(DE3) cells Microbulbifer thermotolerans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.116 Co2+ 53% residual activity at 10 mM Microbulbifer thermotolerans
3.2.1.116 Cu2+ 19% residual activity at 10 mM Microbulbifer thermotolerans
3.2.1.116 EDTA 23% residual activity at 10 mM Microbulbifer thermotolerans
3.2.1.116 Fe3+ 19% residual activity at 10 mM Microbulbifer thermotolerans
3.2.1.116 Hg2+ 17% residual activity at 10 mM Microbulbifer thermotolerans
3.2.1.116 Ni2+ 77% residual activity at 10 mM Microbulbifer thermotolerans
3.2.1.116 Zn2+ 18% residual activity at 10 mM Microbulbifer thermotolerans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.116 additional information not influenced by Mg2+, Ca2+, Cs2+, Li+, and Mn2+ Microbulbifer thermotolerans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.116 80000
-
 x * 80000, SDS-PAGE Microbulbifer thermotolerans

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.116 Acetone 88% activity at 20% (v/v) Microbulbifer thermotolerans
3.2.1.116 benzene 93% activity at 20% (v/v) Microbulbifer thermotolerans
3.2.1.116 chloroform 29% activity at 20% (v/v) Microbulbifer thermotolerans
3.2.1.116 Ethanol 61% activity at 20% (v/v) Microbulbifer thermotolerans
3.2.1.116 hexane the enzyme exhibits extreme stability toward 20% (v/v) hexane (99% activity) Microbulbifer thermotolerans
3.2.1.116 Methanol 56% activity at 20% (v/v) Microbulbifer thermotolerans
3.2.1.116 n-Butanol 51% activity at 20% (v/v) Microbulbifer thermotolerans

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.116 Microbulbifer thermotolerans A0A0A0Q4S7
-
-
3.2.1.116 Microbulbifer thermotolerans DAU221 A0A0A0Q4S7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.116 His-tag affinity column chromatography Microbulbifer thermotolerans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.116 soluble starch + H2O the Km and Vmax values for the hydrolysis of soluble starch are 1.08 mg/ml and 1.736 mM maltotriose/mg protein/min, respectively Microbulbifer thermotolerans maltotriose + ?
-
 ?
3.2.1.116 soluble starch + H2O the Km and Vmax values for the hydrolysis of soluble starch are 1.08 mg/ml and 1.736 mM maltotriose/mg protein/min, respectively Microbulbifer thermotolerans DAU221 maltotriose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.116 ? x * 80000, SDS-PAGE Microbulbifer thermotolerans

Synonyms

EC Number Synonyms Comment Organism
3.2.1.116 AmyA
-
 Microbulbifer thermotolerans
3.2.1.116 maltotriose-producing alpha-amylase
-
 Microbulbifer thermotolerans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.116 50
-
 in sodium phosphate buffer (pH 6.0) Microbulbifer thermotolerans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.116 30 50 at 30°C, the enzyme maintains approximately above 90% activity until 6 h and 80% activity until 12 h. At 40°C, the enzyme maintains approximately above 90% activity until 2 h, 80% activity until 4 h, 70% activity until 8 h, and 60% activity until 12 h. At 50°C, the enzyme maintains approximately 50% activity until 1 h, 30% activity until 6 h, and 20% activity until 12 h Microbulbifer thermotolerans