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Literature summary extracted from

  • Fujita, K.; Sakaguchi, T.; Sakamoto, A.; Shimokawa, M.; Kitahara, K.
    Bifidobacterium longum subsp. longum exo-beta-1,3-galactanase, an enzyme for the degradation of type II arabinogalactan (2014), Appl. Environ. Microbiol., 80, 4577-4584.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.145 BLLJ_1840, phylogenetic tree, expresion as soluble enzyme in Escherichia coli Bifidobacterium longum

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.145 additional information construction of deletion mutants NDELTA42, NDELTA42CDELTA257, NDELTA42CDELTA457, and NDELTA42CDELTA76 showing 100%, 80.1%, 122%, and 8.45% activity relative to wild-type enzyme activity Bifidobacterium longum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.145 additional information
-
 additional information kinetic parameters of enzyme BLLJ_1840 activity on different substrates, overview Bifidobacterium longum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.145 106100
-
 sequence calculation Bifidobacterium longum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.145 Bifidobacterium longum A0A061M1M5 subsp. longum
-
3.2.1.145 Bifidobacterium longum JCM 1217 A0A061M1M5 subsp. longum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.145 0.0139
-
 substrate beta-1,3-D-galactotetraose, pH 5.5, 40°C Bifidobacterium longum
3.2.1.145 0.0157
-
 substrate beta-1,3-D-galactopentaose, pH 5.5, 40°C Bifidobacterium longum
3.2.1.145 0.0188
-
 substrate beta-1,3-D-galactotriose, pH 5.5, 40°C Bifidobacterium longum
3.2.1.145 0.0218
-
 substrate beta-1,3-D-galactobiose, pH 5.5, 40°C Bifidobacterium longum
3.2.1.145 0.113
-
 substrate gum arabic, pH 5.5, 40°C Bifidobacterium longum
3.2.1.145 1.01
-
 substrate beta-1,3-galactan, pH 5.5, 40°C Bifidobacterium longum
3.2.1.145 1.17
-
 substrate larch wood arabinogalactan, pH 5.5, 40°C Bifidobacterium longum
3.2.1.145 1.28
-
 substrate dearabinosylated gum arabic, pH 5.5, 40°C Bifidobacterium longum
3.2.1.145 16.2
-
 substrate dearabinosylated larch wood arabinogalactan, pH 5.5, 40°C Bifidobacterium longum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.145 beta-1,3-D-galactobiose + H2O best beta-1,3-galactan substrate Bifidobacterium longum 2 D-galactose
-
 ?
3.2.1.145 beta-1,3-D-galactobiose + H2O best beta-1,3-galactan substrate Bifidobacterium longum JCM 1217 2 D-galactose
-
 ?
3.2.1.145 beta-1,3-D-galactopentaose + H2O
-
 Bifidobacterium longum D-galactose + ?
-
 ?
3.2.1.145 beta-1,3-D-galactopentaose + H2O
-
 Bifidobacterium longum JCM 1217 D-galactose + ?
-
 ?
3.2.1.145 beta-1,3-D-galactotetraose + H2O
-
 Bifidobacterium longum D-galactose + ?
-
 ?
3.2.1.145 beta-1,3-D-galactotetraose + H2O
-
 Bifidobacterium longum JCM 1217 D-galactose + ?
-
 ?
3.2.1.145 beta-1,3-D-galactotriose + H2O
-
 Bifidobacterium longum D-galactose + ?
-
 ?
3.2.1.145 dearabinosylated gum arabic + H2O
-
 Bifidobacterium longum ?
-
 ?
3.2.1.145 dearabinosylated larch wood arabinogalactan + H2O best substrate Bifidobacterium longum ?
-
 ?
3.2.1.145 gum arabic + H2O
-
 Bifidobacterium longum ?
-
 ?
3.2.1.145 gum arabic + H2O
-
 Bifidobacterium longum JCM 1217 ?
-
 ?
3.2.1.145 larch wood arabinogalactan + H2O
-
 Bifidobacterium longum ?
-
 ?
3.2.1.145 additional information the recombinant BLLJ_1840 expressed in Escherichia coli hydrolyzes beta-1,3-linked galactooligosaccharides but not beta-1,4- and beta-1,6-linked galactooligosaccharides. The enzyme also hydrolyzes larch wood arabinogalactan, which comprises a beta-1,3-linked galactan backbone with beta-1,6-linked galactan side chains. No activity with 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-alpha-D-galactopyranoside, 4-nitrophenyl-beta-D-glucopyranoside, 4-nitrophenyl-beta-D-xylopyranoside, and 4-nitrophenyl-beta-L-arabinopyranoside, substrate specificity, and no activity with potato galactan and laminarin, overview Bifidobacterium longum ?
-
 ?
3.2.1.145 additional information the recombinant BLLJ_1840 expressed in Escherichia coli hydrolyzes beta-1,3-linked galactooligosaccharides but not beta-1,4- and beta-1,6-linked galactooligosaccharides. The enzyme also hydrolyzes larch wood arabinogalactan, which comprises a beta-1,3-linked galactan backbone with beta-1,6-linked galactan side chains. No activity with 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-alpha-D-galactopyranoside, 4-nitrophenyl-beta-D-glucopyranoside, 4-nitrophenyl-beta-D-xylopyranoside, and 4-nitrophenyl-beta-L-arabinopyranoside, substrate specificity, and no activity with potato galactan and laminarin, overview Bifidobacterium longum JCM 1217 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.145 BLLJ_1840
-
 Bifidobacterium longum
3.2.1.145 exo-beta-1,3-galactanase
-
 Bifidobacterium longum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.145 40
-
-
 Bifidobacterium longum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.145 5.5
-
-
 Bifidobacterium longum

Expression

EC Number Organism Comment Expression
3.2.1.145 Bifidobacterium longum the enzyme is strongly induced by larch wood arabinogalactan up

General Information

EC Number General Information Comment Organism
3.2.1.145 evolution the enzyme belongs to the glycoside hydrolase family 43, GH43, phylogenetic relationships of BLLJ_1840 Bifidobacterium longum
3.2.1.145 additional information domain structures of BLLJ_1840 comprising the GH43 catalytic domain, bacterial Ig-like domain, CBM32, and the LPXTG cell wall anchor domain (Anchor), as well as the signal peptide Bifidobacterium longum