EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.1.67 | D210N | isozyme RPG15 mutant, the mutant shows a 5000fold reduced specific activity relative to the wild-type, rendering the enzyme essentially inactive | Rhizopus arrhizus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.67 | D-galacturonate | product inhibition | Rhizopus arrhizus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.67 | additional information | - |
additional information | thermodynamic parameters of a series of galacturonic acid oligomers binding to isozyme RPG15, overview | Rhizopus arrhizus | |
3.2.1.67 | 0.0092 | - |
[(1->4)-alpha-D-galacturonide]3 | isozyme RPG15, pH 4.0, 30°C | Rhizopus arrhizus | |
3.2.1.67 | 0.016 | - |
[(1->4)-alpha-D-galacturonide]2 | isozyme RPG16, pH 4.0, 30°C | Rhizopus arrhizus | |
3.2.1.67 | 0.055 | - |
[(1->4)-alpha-D-galacturonide]2 | isozyme RPG15, pH 4.0, 30°C | Rhizopus arrhizus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.67 | [(1->4)-alpha-D-galacturonide]n + H2O | Rhizopus arrhizus | - |
[(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.67 | Rhizopus arrhizus | Q5W9U0 | two isozymes RPG15 and RPG16 | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.67 | [(1->4)-alpha-D-galacturonide]2 + H2O | - |
Rhizopus arrhizus | 2 D-galacturonate | - |
? | |
3.2.1.67 | [(1->4)-alpha-D-galacturonide]3 + H2O | - |
Rhizopus arrhizus | [(1->4)-alpha-D-galacturonide]2 + D-galacturonate | - |
? | |
3.2.1.67 | [(1->4)-alpha-D-galacturonide]n + H2O | - |
Rhizopus arrhizus | [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate | - |
? | |
3.2.1.67 | [(1->4)-alpha-D-galacturonide]n + H2O | the two Rhizopus oryzae exo-polygalacturonase isozymes act on galacturonic acid oligomers, isothermal titration calorimetry analysis, overview | Rhizopus arrhizus | [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.67 | Exo-PG | - |
Rhizopus arrhizus |
3.2.1.67 | exo-polygalacturonase | - |
Rhizopus arrhizus |
3.2.1.67 | RPG15 | - |
Rhizopus arrhizus |
3.2.1.67 | RPG16 | - |
Rhizopus arrhizus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.67 | 30 | - |
assay at | Rhizopus arrhizus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.67 | 3.9 | - |
[(1->4)-alpha-D-galacturonide]2 | isozyme RPG16, pH 4.0, 30°C | Rhizopus arrhizus | |
3.2.1.67 | 10.3 | - |
[(1->4)-alpha-D-galacturonide]2 | isozyme RPG15, pH 4.0, 30°C | Rhizopus arrhizus | |
3.2.1.67 | 10.7 | - |
[(1->4)-alpha-D-galacturonide]3 | isozyme RPG15, pH 4.0, 30°C | Rhizopus arrhizus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.67 | 4 | - |
assay at | Rhizopus arrhizus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.67 | 0.501 | - |
D-galacturonate | isozyme RPG16, pH 4.0, 30°C | Rhizopus arrhizus | |
3.2.1.67 | 0.886 | - |
D-galacturonate | isozyme RPG15, pH 4.0, 30°C | Rhizopus arrhizus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.2.1.67 | evolution | the enzyme belongs to the glycosyl hydrolase 28 family, GH28 | Rhizopus arrhizus |