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Literature summary extracted from

  • Bak, H.J.; Kim, M.S.; Kim, N.Y.; Go, H.J.; Han, J.W.; In Jo, H.; Ahn, S.J.; Park, N.G.; Chung, J.K.; Lee, H.H.
    Molecular cloning, expression, and enzymatic analysis of cathepsin X from starfish (Asterina pectinifera) (2013), Appl. Biochem. Biotechnol., 169, 847-861.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.18.1 Brij 35 0.01%, 125% of initial activity Patiria pectinifera
3.4.18.1 KCl 1 mM, 116% of initial activity Patiria pectinifera
3.4.18.1 Triton X-100 0.01%, 123% of initial activity Patiria pectinifera
3.4.18.1 Tween 20 0.01%, 130% of initial activity Patiria pectinifera

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.18.1 expression in Escherichia coli Patiria pectinifera

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.18.1 1,10-phenanthroline 0.1 mM, 89% inhibition Patiria pectinifera
3.4.18.1 antipain 0.1 mM, 62% inhibition Patiria pectinifera
3.4.18.1 Co2+ 1 mM, 71% inhibition Patiria pectinifera
3.4.18.1 Cu2+ 1 mM, 93% inhibition Patiria pectinifera
3.4.18.1 EDTA 0.1 mM, 86% inhibition Patiria pectinifera
3.4.18.1 EGTA 0.1 mM, 84% inhibition Patiria pectinifera
3.4.18.1 Hg2+ 1 mM, 90% inhibition Patiria pectinifera
3.4.18.1 N-ethylmaleimide 0.1 mM, 86% inhibition Patiria pectinifera
3.4.18.1 sodium dodecylsulfate 0.01%, 15.6% residual activity Patiria pectinifera

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.18.1 32700
-
 x * 32700, calculated Patiria pectinifera

Organism

EC Number Organism UniProt Comment Textmining
3.4.18.1 Patiria pectinifera G1JRK2
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.18.1 proteolytic modification
-
 Patiria pectinifera

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.18.1 liver most abundant expression Patiria pectinifera
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.18.1 Ala-Ala-Phe-7-amido-4-methylcoumarin + H2O 61% of the activity with benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin Patiria pectinifera Ala-Ala-Phe + 7-amino-4-methylcoumarin
-
 ?
3.4.18.1 benzyloxycarbonyl-Arg-Arg-7-amido-4-methylcoumarin + H2O 34% of the activity with benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin Patiria pectinifera benzyloxycarbonyl-Arg-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.18.1 benzyloxycarbonyl-Leu-Leu-Glu-7-amido-4-methylcoumarin + H2O 54% of the activity with benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin Patiria pectinifera benzyloxycarbonyl-Leu-Leu-Glu + 7-amino-4-methylcoumarin
-
 ?
3.4.18.1 benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin + H2O
-
 Patiria pectinifera benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.18.1 benzyloxycarbonyl-Val-Val-Arg-7-amido-4-methylcoumarin + H2O 12% of the activity with benzyloxycarbonyl-Phe-Arg-7-amido-4-methylcoumarin Patiria pectinifera benzyloxycarbonyl-Val-Val-Arg + 7-amino-4-methylcoumarin
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.18.1 ? x * 32700, calculated Patiria pectinifera

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.18.1 6.5
-
-
 Patiria pectinifera

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.18.1 6 8
-
 Patiria pectinifera