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Literature summary extracted from
- Structure of a novel alpha-amylase AmyB from Thermotoga neapolitana that produces maltose from the nonreducing end of polysaccharides (2013), Acta Crystallogr. Sect. D, 69, 442-450.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.133 | expressed in Escherichia coli BL21(DE3) cells | Thermotoga neapolitana |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.2.1.133 | sitting drop vapor diffusion method | Thermotoga neapolitana |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.2.1.133 | D135A | inactive | Thermotoga neapolitana |
| 3.2.1.133 | D169N | inactive | Thermotoga neapolitana |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.133 | additional information | not inhibited by acarbose | Thermotoga neapolitana |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.133 | 50000 | - |
x * 50000, SDS-PAGE | Thermotoga neapolitana |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.133 | Thermotoga neapolitana | B5ARZ9 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.133 | HiTrap SP column chromatography and Superdex 200 gel filtration | Thermotoga neapolitana |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.133 | 4-nitrophenyl beta-D-glucoside + H2O | poor substrate | Thermotoga neapolitana | 4-nitrophenol + D-glucose | - |
? |  |
| 3.2.1.133 | 4-nitrophenyl maltopyranoside + H2O | excellent substrate | Thermotoga neapolitana | 4-nitrophenol + maltose | - |
? | |
| 3.2.1.133 | amylopectin + H2O | - |
Thermotoga neapolitana | maltose + ? | - |
? | |
| 3.2.1.133 | amylose + H2O | - |
Thermotoga neapolitana | maltose + ? | - |
? | |
| 3.2.1.133 | glycogen + H2O | - |
Thermotoga neapolitana | maltose + ? | - |
? | |
| 3.2.1.133 | maltotriose + H2O | - |
Thermotoga neapolitana | maltose + D-glucose | - |
? | |
| 3.2.1.133 | additional information | does not hydrolyze cyclodextrin, pullulan and acarbose | Thermotoga neapolitana | ? | - |
? | |
| 3.2.1.133 | starch + H2O | - |
Thermotoga neapolitana | maltose + ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.133 | ? | x * 50000, SDS-PAGE | Thermotoga neapolitana |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.133 | alpha-amylase | - |
Thermotoga neapolitana |
| 3.2.1.133 | AmyB | - |
Thermotoga neapolitana |
| 3.2.1.133 | maltogenic amylase | - |
Thermotoga neapolitana |
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Release 2023.1 (January 2023)
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