Literature summary extracted from
Wojtkowiak, A.; Witek, K.; Hennig, J.; Jaskolski, M.
Two high-resolution structures of potato endo-1,3-beta-glucanase reveal subdomain flexibility with implications for substrate binding (2012), Acta Crystallogr. Sect. D, 68, 713-723.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.2.1.39 |
gene gluB20-2, expression in Escherichia coli strain BL21 |
Solanum tuberosum |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
3.2.1.39 |
two different crystal forms: higher density and lower density determined at 1.40 A and 1.26 A resolution, respectively. The crystal structures reveal an unusual packing mode, clearly visible in the LD structure, caused by the presence of the C-terminal His6 tag, which extends from the compact fold of the enzyme molecule and docks in the catalytic cleft of a neighbouring molecule. In this way, an infinite chain of His-tag-linked protein molecules is formed along the c direction. Purified recombinant His-tagged enzyme by hanging drop vapour diffusion method, mixing of 0.0015 ml of 8 mg/ml protein in 20 mM Tris-HCl, pH 8.0, with 0.0015 ml of reservoir solution containing 0.1 M sodium acetate, pH 4.6, 0.2 M ammonium acetate, 25-30% PEG 4000, 21°C, 2 days, X-ray diffraction structure determination and analysis, modelling |
Solanum tuberosum |
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
3.2.1.39 |
additional information |
the enzyme has a 23-residue signal peptide at the N-terminus |
Solanum tuberosum |
- |
- |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.2.1.39 |
Solanum tuberosum |
Q70C53 |
gene gluB20-2 |
- |
3.2.1.39 |
Solanum tuberosum Desiree |
Q70C53 |
gene gluB20-2 |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.2.1.39 |
recombinant enzyme from Escherichia coli strain BL21 cell-free extract by ammonium sulfate fractionation, anion exchange chromatography, nickel affinity chromatography, and dialysis |
Solanum tuberosum |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.2.1.39 |
endo-1,3-beta-glucanase |
- |
Solanum tuberosum |
General Information
EC Number |
General Information |
Comment |
Organism |
---|
3.2.1.39 |
evolution |
the enzyme belongs to the glycosyl hydrolase family 17, GH17 |
Solanum tuberosum |