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Literature summary extracted from

  • Xavier, K.B.; Miller, S.T.; Lu, W.; Kim, J.H.; Rabinowitz, J.; Pelczer, I.; Semmelhack, M.F.; Bassler, B.L.
    Phosphorylation and processing of the quorum-sensing molecule autoinducer-2 in enteric bacteria (2007), ACS Chem. Biol., 2, 128-136.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.189 gene lsrK, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21 Salmonella enterica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.189 Mg2+ required Salmonella enterica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.189 ATP + 4,5-dihydroxypentane-2,3-dione Salmonella enterica the kinase LsrK phosphorylates carbon-5 of the open form of the substrate, molecule (S)-4,5-dihydroxypentane-2,3-dione is the precursor of the signal molecule autoinducer-2 ADP + 2-hydroxy-3,4-dioxopentyl phosphate
-
 ?
2.7.1.189 ATP + 4,5-dihydroxypentane-2,3-dione Salmonella enterica serovar Typhimurium the kinase LsrK phosphorylates carbon-5 of the open form of the substrate, molecule (S)-4,5-dihydroxypentane-2,3-dione is the precursor of the signal molecule autoinducer-2 ADP + 2-hydroxy-3,4-dioxopentyl phosphate
-
 ?
2.7.1.189 ATP + autoinducer-2 Salmonella enterica
-
 ADP + phosphorylated autoinducer-2
-
 ?
2.7.1.189 ATP + autoinducer-2 Salmonella enterica serovar Typhimurium
-
 ADP + phosphorylated autoinducer-2
-
 ?
5.3.1.32 (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione Salmonella enterica (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione i.e. autoinducer 2 i.e. AI-2. The enzyme participates in a degradation pathway of the bacterial quorum-sensing autoinducer molecule AI-2 3-hydroxy-5-phosphonooxypentane-2,4-dione
-
 ?
5.3.1.32 (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione Salmonella enterica serovar Typhimurium (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione i.e. autoinducer 2 i.e. AI-2. The enzyme participates in a degradation pathway of the bacterial quorum-sensing autoinducer molecule AI-2 3-hydroxy-5-phosphonooxypentane-2,4-dione
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.189 Salmonella enterica
-
 serovar Typhimurium, gene lsrK
-
5.3.1.32 Salmonella enterica
-
-
-
5.3.1.32 Salmonella enterica serovar Typhimurium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.189 recombinant His6-tagged enzyme from Escherichia coli strain BL21 to homogeneity by nickel affinity chromatography and gel filtration Salmonella enterica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.189 ATP + 4,5-dihydroxypentane-2,3-dione the kinase LsrK phosphorylates carbon-5 of the open form of the substrate, molecule (S)-4,5-dihydroxypentane-2,3-dione is the precursor of the signal molecule autoinducer-2 Salmonella enterica ADP + 2-hydroxy-3,4-dioxopentyl phosphate
-
 ?
2.7.1.189 ATP + 4,5-dihydroxypentane-2,3-dione the kinase LsrK phosphorylates carbon-5 of the open form of the substrate, mass spectrometric product identification. Molecule (S)-4,5-dihydroxypentane-2,3-dione (DPD) is the precursor of the signal molecule autoinducer-2 Salmonella enterica ADP + 2-hydroxy-3,4-dioxopentyl phosphate
-
 ?
2.7.1.189 ATP + 4,5-dihydroxypentane-2,3-dione the kinase LsrK phosphorylates carbon-5 of the open form of the substrate, molecule (S)-4,5-dihydroxypentane-2,3-dione is the precursor of the signal molecule autoinducer-2 Salmonella enterica serovar Typhimurium ADP + 2-hydroxy-3,4-dioxopentyl phosphate
-
 ?
2.7.1.189 ATP + 4,5-dihydroxypentane-2,3-dione the kinase LsrK phosphorylates carbon-5 of the open form of the substrate, mass spectrometric product identification. Molecule (S)-4,5-dihydroxypentane-2,3-dione (DPD) is the precursor of the signal molecule autoinducer-2 Salmonella enterica serovar Typhimurium ADP + 2-hydroxy-3,4-dioxopentyl phosphate
-
 ?
2.7.1.189 ATP + autoinducer-2
-
 Salmonella enterica ADP + phosphorylated autoinducer-2
-
 ?
2.7.1.189 ATP + autoinducer-2 i.e. AI-2 Salmonella enterica ADP + phosphorylated autoinducer-2
-
 ?
2.7.1.189 ATP + autoinducer-2
-
 Salmonella enterica serovar Typhimurium ADP + phosphorylated autoinducer-2
-
 ?
5.3.1.32 (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione i.e. autoinducer 2 i.e. AI-2. The enzyme participates in a degradation pathway of the bacterial quorum-sensing autoinducer molecule AI-2 Salmonella enterica 3-hydroxy-5-phosphonooxypentane-2,4-dione
-
 ?
5.3.1.32 (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione i.e. autoinducer 2 i.e. AI-2 Salmonella enterica 3-hydroxy-5-phosphonooxypentane-2,4-dione
-
 ?
5.3.1.32 (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione i.e. autoinducer 2 i.e. AI-2. The enzyme participates in a degradation pathway of the bacterial quorum-sensing autoinducer molecule AI-2 Salmonella enterica serovar Typhimurium 3-hydroxy-5-phosphonooxypentane-2,4-dione
-
 ?
5.3.1.32 (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione (4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione i.e. autoinducer 2 i.e. AI-2 Salmonella enterica serovar Typhimurium 3-hydroxy-5-phosphonooxypentane-2,4-dione
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.189 lsrK
-
 Salmonella enterica
5.3.1.32 lsrG
-
 Salmonella enterica
5.3.1.32 phospho-AI-2 isomerase
-
 Salmonella enterica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.189 37
-
 assay at Salmonella enterica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.189 7.5
-
 assay at Salmonella enterica

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.189 ATP
-
 Salmonella enterica

Expression

EC Number Organism Comment Expression
2.7.1.189 Salmonella enterica phosphorylated 4,5-dihydroxy-2,3-pentanedione (P-DPD) binds specifically to the repressor of the lsr operon, LsrR, consistent with P-DPD being the inducer of the lsr operon up

General Information

EC Number General Information Comment Organism
2.7.1.189 physiological function enteric bacteria respond to the presence of competitor bacteria by sequestering and destroying autoinducer-2, through phosphorylation by the enzyme LsrK and cleavage of 4-hydroxy-2,3-pentanedione 5-phosphate producing 2-phosphoglycolic acid via enzyme LsrG, thereby eliminating the competitors' intercellular communication capabilities. The phosphorylated form of AI-2 is the intracellular signal responsible for lsr activation Salmonella enterica
5.3.1.32 metabolism the enzyme participates in a degradation pathway of the bacterial quorum-sensing autoinducer molecule AI-2 Salmonella enterica