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Literature summary extracted from

  • Mozzi, A.; Mazzacuva, P.; Zampella, G.; Forcella, M.E.; Fusi, P.A.; Monti, E.
    Molecular insight into substrate recognition by human cytosolic sialidase NEU2 (2012), Proteins, 80, 1123-1132.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.18 expressed as GST-fusion protein in Escherichia coli BL21(DE3) cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.18 D46A inactive Homo sapiens
3.2.1.18 E218A inactive Homo sapiens
3.2.1.18 E218Q inactive Homo sapiens
3.2.1.18 K45A the mutant shows about 3fold increased activity towards 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid compared to the wild type Homo sapiens
3.2.1.18 Q112A the mutant shows nearly 4fold increased activity towards 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid compared to the wild type Homo sapiens
3.2.1.18 Q270A the mutant shows about 4fold increased activity towards 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid compared to the wild type Homo sapiens
3.2.1.18 Q270E the mutant shows about wild type activity Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.18 cytosol
-
 Homo sapiens 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.18 alpha(2-3)sialyl-lactose + H2O Homo sapiens
-
 ?
-
 ?
3.2.1.18 monosialoganglioside GM3 + H2O Homo sapiens
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.18 Homo sapiens Q9Y3R4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.18 glutathione-Sepharose 4B column chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.18 4-methylumbelliferyl-alpha-D-N-acetylneuraminic acid + H2O
-
 Homo sapiens 4-methylumbelliferone + N-acetylneuraminic acid
-
 ?
3.2.1.18 alpha(2-3)sialyl-lactose + H2O
-
 Homo sapiens ?
-
 ?
3.2.1.18 monosialoganglioside GM3 + H2O
-
 Homo sapiens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.18 N-acylneuraminosyl glycohydrolase
-
 Homo sapiens
3.2.1.18 NEU2
-
 Homo sapiens
3.2.1.18 sialidase
-
 Homo sapiens