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Literature summary extracted from

  • Nguyen, H.; Nguyen, T.; Nguyen, T.; Peterbauer, C.; Mathiesen, G.; Haltrich, D.
    Chitinase from Bacillus licheniformis DSM13: expression in Lactobacillus plantarum WCFS1 and biochemical characterisation (2012), Protein Expr. Purif., 81, 166-174.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.14 gene chi, cloning in Escherichia coli strain NEB 5alpha, recombinant expression of His-tagged enzyme in Lactobacillus plantarum strain WCFS1 using inducible lactobacillal expression vectors pSIP403 and pSIP409, derived from the sakacin-P operon of Lactobacillus sakei Bacillus licheniformis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 65000
-
 1 * 65000, recombinant His-tagged enzyme, SDS-PAGE Bacillus licheniformis
3.2.1.14 66500
-
 recombinant His-tagged enzyme, gel filtration Bacillus licheniformis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Bacillus licheniformis A5A699 gene CHI
-
3.2.1.14 Bacillus licheniformis DSM 13 A5A699 gene CHI
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 recombinant His-tagged enzyme from Lactobacillus plantarum strain WCFS1 by immobilized metal affinity chromatography Bacillus licheniformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 chitin + H2O from crab shells Bacillus licheniformis ?
-
 ?
3.2.1.14 chitin + H2O from crab shells Bacillus licheniformis DSM 13 ?
-
 ?
3.2.1.14 chitosan + H2O
-
 Bacillus licheniformis ?
-
 ?
3.2.1.14 chitosan + H2O
-
 Bacillus licheniformis DSM 13 ?
-
 ?
3.2.1.14 N,N'-diacetylchitobiose + H2O
-
 Bacillus licheniformis deacetylated chitooligosaccharides + acetate
-
 ?
3.2.1.14 N,N'-diacetylchitobiose + H2O
-
 Bacillus licheniformis DSM 13 deacetylated chitooligosaccharides + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 monomer 1 * 65000, recombinant His-tagged enzyme, SDS-PAGE Bacillus licheniformis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 50
-
 recombinant His-tagged enzyme Bacillus licheniformis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.14 37
-
 purified recombinant enzyme, half-life is 20 days Bacillus licheniformis
3.2.1.14 50
-
 purified recombinant enzyme, half-life is 8.4 days Bacillus licheniformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 7.5
-
 recombinant His-tagged enzyme Bacillus licheniformis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.14 5 10 purified recombinant enzyme, stable at, over 80% activity remaining Bacillus licheniformis

General Information

EC Number General Information Comment Organism
3.2.1.14 evolution the enzyme belongs to the glycosyl hydrolase family GH18 Bacillus licheniformis