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Literature summary extracted from

  • Chen, H.; Tseng, C.C.; Hubbard, B.K.; Walsh, C.T.
    Glycopeptide antibiotic biosynthesis: enzymatic assembly of the dedicated amino acid monomer (S)-3,5-dihydroxyphenylglycine (2001), Proc. Natl. Acad. Sci. USA, 98, 14901-14906.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.80 expression in Escherichia coli Amycolatopsis orientalis
2.3.1.246 expressed in Escherichia coli Amycolatopsis orientalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.80 0.006
-
 (3,5-dihydroxyphenyl)acetyl-CoA pH 7.5, 24°C Amycolatopsis orientalis
1.13.11.80 0.3
-
 phenylacetyl-CoA pH 7.5, 24°C Amycolatopsis orientalis

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.80 Amycolatopsis orientalis G4V4T6
-
-
2.3.1.246 Amycolatopsis orientalis
-
-
-
2.3.1.246 Amycolatopsis orientalis NRRL 18098
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.80
-
 Amycolatopsis orientalis
2.3.1.246
-
 Amycolatopsis orientalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.80 (3,5-dihydroxyphenyl)acetyl-CoA + O2
-
 Amycolatopsis orientalis (3,5-dihydroxyphenyl)glyoxylate + CoA
-
 ?
1.13.11.80 phenylacetyl-CoA + O2
-
 Amycolatopsis orientalis phenylglyoxylate + CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.80 DpgC
-
 Amycolatopsis orientalis
2.3.1.246 dpgA
-
 Amycolatopsis orientalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.80 24
-
 assay at Amycolatopsis orientalis
2.3.1.246 24
-
 assay at Amycolatopsis orientalis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.80 0.017
-
 phenylacetyl-CoA pH 7.5, 24°C Amycolatopsis orientalis
1.13.11.80 0.17
-
 (3,5-dihydroxyphenyl)acetyl-CoA pH 7.5, 24°C Amycolatopsis orientalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.80 7.5
-
 assay at Amycolatopsis orientalis
2.3.1.246 7.5
-
 assay at Amycolatopsis orientalis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.13.11.80 0.06
-
 phenylacetyl-CoA pH 7.5, 24°C Amycolatopsis orientalis
1.13.11.80 28.3
-
 (3,5-dihydroxyphenyl)acetyl-CoA pH 7.5, 24°C Amycolatopsis orientalis