Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kovacic, F.; Granzin, J.; Wilhelm, S.; Kojic-Prodic, B.; Batra-Safferling, R.; Jaeger, K.E.
    Structural and functional characterisation of TesA - a novel lysophospholipase A from Pseudomonas aeruginosa (2013), PLoS ONE, 8, e69125.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.5 expressed in Escherichia coli BL21(DE3) cells Pseudomonas aeruginosa

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.5 sitting drop vapor diffusion method, using 20% (w/v) PEG 3350, 50 mM sodium-citrate buffer, pH 4.5 Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.5 D17S/L162R the mutant shows 2.2fold increased thioesterase activity compared to the wild type Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.5 1.78
-
 1-lauryl-glycerophosphocholine at pH 8.0 and 37°C Pseudomonas aeruginosa
3.1.1.5 2.04
-
 1-palmitoyl-glycerophosphocholine at pH 8.0 and 37°C Pseudomonas aeruginosa
3.1.1.5 7.22
-
 1-hexyl-glycerophosphocholine at pH 8.0 and 37°C Pseudomonas aeruginosa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.5 periplasm
-
 Pseudomonas aeruginosa
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.5 20000
-
 x * 20000, SDS-PAGE Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.5 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.5 Ni-NTA column chromatography Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.5 1-hexyl-glycerophosphocholine + H2O
-
 Pseudomonas aeruginosa hexanoate + glycerophosphocholine
-
 ?
3.1.1.5 1-lauryl-glycerophosphocholine + H2O
-
 Pseudomonas aeruginosa laurate + glycerophosphocholine
-
 ?
3.1.1.5 1-oleoyl-glycerophosphocholine + H2O
-
 Pseudomonas aeruginosa oleate + glycerophosphocholine
-
 ?
3.1.1.5 1-palmitoyl-glycerophosphocholine + H2O
-
 Pseudomonas aeruginosa palmitate + glycerophosphocholine
-
 ?
3.1.1.5 1-stearoyl-glycerophosphocholine + H2O
-
 Pseudomonas aeruginosa stearate + glycerophosphocholine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.5 ? x * 20000, SDS-PAGE Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
3.1.1.5 lysophospholipase A
-
 Pseudomonas aeruginosa
3.1.1.5 TesA
-
 Pseudomonas aeruginosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.5 921.1
-
 1-palmitoyl-glycerophosphocholine at pH 8.0 and 37°C Pseudomonas aeruginosa
3.1.1.5 1016
-
 1-hexyl-glycerophosphocholine at pH 8.0 and 37°C Pseudomonas aeruginosa
3.1.1.5 1975
-
 1-lauryl-glycerophosphocholine at pH 8.0 and 37°C Pseudomonas aeruginosa

General Information

EC Number General Information Comment Organism
3.1.1.5 metabolism the enzyme exerts important functions in the homeostasis of phospholipids or detoxification of lysophospholipids Pseudomonas aeruginosa

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.1.5 110
-
 1-hexyl-glycerophosphocholine at pH 8.0 and 37°C Pseudomonas aeruginosa
3.1.1.5 450
-
 1-palmitoyl-glycerophosphocholine at pH 8.0 and 37°C Pseudomonas aeruginosa
3.1.1.5 1100
-
 1-lauryl-glycerophosphocholine at pH 8.0 and 37°C Pseudomonas aeruginosa