BRENDA - Enzyme Database

Borrelia burgdorferi enolase is a surface-exposed plasminogen binding protein

Floden, A.M.; Watt, J.A.; Brissette, C.A.; PLoS ONE 6, e27502 (2011)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.11
expressed in Escherichia coli Rosetta (DE3)pLysS cells
Borreliella burgdorferi
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.11
cytoplasm
-
Borreliella burgdorferi
5737
-
4.2.1.11
outer surface
-
Borreliella burgdorferi
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Mn2+
required
Borreliella burgdorferi
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
50000
-
x * 50000, SDS-PAGE
Borreliella burgdorferi
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Borreliella burgdorferi
-
phosphoenolpyruvate + H2O
-
-
?
4.2.1.11
2-phospho-D-glycerate
Borreliella burgdorferi B31-MI-16
-
phosphoenolpyruvate + H2O
-
-
?
4.2.1.11
additional information
Borreliella burgdorferi
plasminogen bound to recombinant enolase can be converted to active plasmin
?
-
-
-
4.2.1.11
additional information
Borreliella burgdorferi B31-MI-16
plasminogen bound to recombinant enolase can be converted to active plasmin
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Borreliella burgdorferi
-
-
-
4.2.1.11
Borreliella burgdorferi B31-MI-16
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
MagneHis nickel-conjugated magnetic bead column chromatography, and gel filtration
Borreliella burgdorferi
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
730683
Borreliella burgdorferi
phosphoenolpyruvate + H2O
-
-
-
?
4.2.1.11
2-phospho-D-glycerate
-
730683
Borreliella burgdorferi B31-MI-16
phosphoenolpyruvate + H2O
-
-
-
?
4.2.1.11
additional information
plasminogen bound to recombinant enolase can be converted to active plasmin
730683
Borreliella burgdorferi
?
-
-
-
-
4.2.1.11
additional information
plasminogen bound to recombinant enolase can be converted to active plasmin
730683
Borreliella burgdorferi B31-MI-16
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.11
?
x * 50000, SDS-PAGE
Borreliella burgdorferi
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
4.2.1.11
Borreliella burgdorferi
calculated from amino acid sequence
-
5.4
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
expressed in Escherichia coli Rosetta (DE3)pLysS cells
Borreliella burgdorferi
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.11
cytoplasm
-
Borreliella burgdorferi
5737
-
4.2.1.11
outer surface
-
Borreliella burgdorferi
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Mn2+
required
Borreliella burgdorferi
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
50000
-
x * 50000, SDS-PAGE
Borreliella burgdorferi
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Borreliella burgdorferi
-
phosphoenolpyruvate + H2O
-
-
?
4.2.1.11
2-phospho-D-glycerate
Borreliella burgdorferi B31-MI-16
-
phosphoenolpyruvate + H2O
-
-
?
4.2.1.11
additional information
Borreliella burgdorferi
plasminogen bound to recombinant enolase can be converted to active plasmin
?
-
-
-
4.2.1.11
additional information
Borreliella burgdorferi B31-MI-16
plasminogen bound to recombinant enolase can be converted to active plasmin
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
MagneHis nickel-conjugated magnetic bead column chromatography, and gel filtration
Borreliella burgdorferi
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
730683
Borreliella burgdorferi
phosphoenolpyruvate + H2O
-
-
-
?
4.2.1.11
2-phospho-D-glycerate
-
730683
Borreliella burgdorferi B31-MI-16
phosphoenolpyruvate + H2O
-
-
-
?
4.2.1.11
additional information
plasminogen bound to recombinant enolase can be converted to active plasmin
730683
Borreliella burgdorferi
?
-
-
-
-
4.2.1.11
additional information
plasminogen bound to recombinant enolase can be converted to active plasmin
730683
Borreliella burgdorferi B31-MI-16
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.11
?
x * 50000, SDS-PAGE
Borreliella burgdorferi
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
4.2.1.11
Borreliella burgdorferi
calculated from amino acid sequence
-
5.4
General Information
EC Number
General Information
Commentary
Organism
4.2.1.11
physiological function
enolase is a moonlighting cytoplasmic protein which also associates with the bacterial outer surface and facilitates binding to host plasminogen
Borreliella burgdorferi
General Information (protein specific)
EC Number
General Information
Commentary
Organism
4.2.1.11
physiological function
enolase is a moonlighting cytoplasmic protein which also associates with the bacterial outer surface and facilitates binding to host plasminogen
Borreliella burgdorferi