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Literature summary extracted from

  • Floden, A.M.; Watt, J.A.; Brissette, C.A.
    Borrelia burgdorferi enolase is a surface-exposed plasminogen binding protein (2011), PLoS ONE, 6, e27502.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.11 expressed in Escherichia coli Rosetta (DE3)pLysS cells Borreliella burgdorferi

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.11 cytoplasm
-
 Borreliella burgdorferi 5737
-
4.2.1.11 outer surface
-
 Borreliella burgdorferi
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.11 Mn2+ required Borreliella burgdorferi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.11 50000
-
 x * 50000, SDS-PAGE Borreliella burgdorferi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.11 2-phospho-D-glycerate Borreliella burgdorferi
-
 phosphoenolpyruvate + H2O
-
 ?
4.2.1.11 2-phospho-D-glycerate Borreliella burgdorferi B31-MI-16
-
 phosphoenolpyruvate + H2O
-
 ?
4.2.1.11 additional information Borreliella burgdorferi plasminogen bound to recombinant enolase can be converted to active plasmin ?
-
 ?
4.2.1.11 additional information Borreliella burgdorferi B31-MI-16 plasminogen bound to recombinant enolase can be converted to active plasmin ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.11 Borreliella burgdorferi
-
-
-
4.2.1.11 Borreliella burgdorferi B31-MI-16
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.11 MagneHis nickel-conjugated magnetic bead column chromatography, and gel filtration Borreliella burgdorferi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.11 2-phospho-D-glycerate
-
 Borreliella burgdorferi phosphoenolpyruvate + H2O
-
 ?
4.2.1.11 2-phospho-D-glycerate
-
 Borreliella burgdorferi B31-MI-16 phosphoenolpyruvate + H2O
-
 ?
4.2.1.11 additional information plasminogen bound to recombinant enolase can be converted to active plasmin Borreliella burgdorferi ?
-
 ?
4.2.1.11 additional information plasminogen bound to recombinant enolase can be converted to active plasmin Borreliella burgdorferi B31-MI-16 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.11 ? x * 50000, SDS-PAGE Borreliella burgdorferi

Synonyms

EC Number Synonyms Comment Organism
4.2.1.11 enolase
-
 Borreliella burgdorferi

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.1.11 Borreliella burgdorferi calculated from amino acid sequence
-
 5.4

General Information

EC Number General Information Comment Organism
4.2.1.11 physiological function enolase is a moonlighting cytoplasmic protein which also associates with the bacterial outer surface and facilitates binding to host plasminogen Borreliella burgdorferi