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Literature summary extracted from

  • Sommer, M.R.; Jochimsen, B.
    Identification of enzymes involved in indole-3-acetic acid degradation (1996), Plant Soil, 186, 143-149.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.20 0.011
-
 isatin pH 9.0, 32°C Mesorhizobium loti

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.2.20 isatin + H2O Bradyrhizobium japonicum the enzyme is involved in the degradation of indole-3-acetic acid isatinate
-
 ?
3.5.2.20 isatin + H2O Mesorhizobium loti the enzyme is involved in the degradation of indole-3-acetic acid isatinate
-
 ?
3.5.2.20 isatin + H2O Neorhizobium galegae the enzyme is involved in the degradation of indole-3-acetic acid isatinate
-
 ?
3.5.2.20 isatin + H2O Bradyrhizobium japonicum USDA122 the enzyme is involved in the degradation of indole-3-acetic acid isatinate
-
 ?
3.5.2.20 isatin + H2O Bradyrhizobium japonicum 61A24 the enzyme is involved in the degradation of indole-3-acetic acid isatinate
-
 ?
3.5.2.20 isatin + H2O Mesorhizobium loti NZP 2037 the enzyme is involved in the degradation of indole-3-acetic acid isatinate
-
 ?
3.5.2.20 isatin + H2O Bradyrhizobium japonicum 110 the enzyme is involved in the degradation of indole-3-acetic acid isatinate
-
 ?
3.5.2.20 isatin + H2O Neorhizobium galegae HAMBI 540 the enzyme is involved in the degradation of indole-3-acetic acid isatinate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.20 Bradyrhizobium japonicum
-
-
-
3.5.2.20 Bradyrhizobium japonicum 110
-
-
-
3.5.2.20 Bradyrhizobium japonicum 61A24
-
-
-
3.5.2.20 Bradyrhizobium japonicum USDA122
-
-
-
3.5.2.20 Mesorhizobium loti
-
-
-
3.5.2.20 Mesorhizobium loti NZP 2037
-
-
-
3.5.2.20 Neorhizobium galegae
-
-
-
3.5.2.20 Neorhizobium galegae HAMBI 540
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.20 partial Mesorhizobium loti

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.2.20 0.34
-
 pH 9.0, 32°C Bradyrhizobium japonicum
3.5.2.20 0.41
-
 pH 9.0, 32°C Bradyrhizobium japonicum
3.5.2.20 0.611
-
 pH 9.0, 32°C Bradyrhizobium japonicum
3.5.2.20 0.64
-
 pH 9.0, 32°C Mesorhizobium loti
3.5.2.20 0.69
-
 pH 9.0, 32°C Neorhizobium galegae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.20 isatin + H2O the enzyme is involved in the degradation of indole-3-acetic acid Bradyrhizobium japonicum isatinate
-
 ?
3.5.2.20 isatin + H2O the enzyme is involved in the degradation of indole-3-acetic acid Mesorhizobium loti isatinate
-
 ?
3.5.2.20 isatin + H2O the enzyme is involved in the degradation of indole-3-acetic acid Neorhizobium galegae isatinate
-
 ?
3.5.2.20 isatin + H2O isatin i.e. 1H-indole-2,3-dione Bradyrhizobium japonicum isatinate isatinate i.e. 2-(2-aminophenyl)-2-oxoacetate ?
3.5.2.20 isatin + H2O isatin i.e. 1H-indole-2,3-dione Mesorhizobium loti isatinate isatinate i.e. 2-(2-aminophenyl)-2-oxoacetate ?
3.5.2.20 isatin + H2O isatin i.e. 1H-indole-2,3-dione Neorhizobium galegae isatinate isatinate i.e. 2-(2-aminophenyl)-2-oxoacetate ?
3.5.2.20 isatin + H2O the enzyme is involved in the degradation of indole-3-acetic acid Bradyrhizobium japonicum USDA122 isatinate
-
 ?
3.5.2.20 isatin + H2O isatin i.e. 1H-indole-2,3-dione Bradyrhizobium japonicum USDA122 isatinate isatinate i.e. 2-(2-aminophenyl)-2-oxoacetate ?
3.5.2.20 isatin + H2O the enzyme is involved in the degradation of indole-3-acetic acid Bradyrhizobium japonicum 61A24 isatinate
-
 ?
3.5.2.20 isatin + H2O isatin i.e. 1H-indole-2,3-dione Bradyrhizobium japonicum 61A24 isatinate isatinate i.e. 2-(2-aminophenyl)-2-oxoacetate ?
3.5.2.20 isatin + H2O the enzyme is involved in the degradation of indole-3-acetic acid Mesorhizobium loti NZP 2037 isatinate
-
 ?
3.5.2.20 isatin + H2O isatin i.e. 1H-indole-2,3-dione Mesorhizobium loti NZP 2037 isatinate isatinate i.e. 2-(2-aminophenyl)-2-oxoacetate ?
3.5.2.20 isatin + H2O the enzyme is involved in the degradation of indole-3-acetic acid Bradyrhizobium japonicum 110 isatinate
-
 ?
3.5.2.20 isatin + H2O isatin i.e. 1H-indole-2,3-dione Bradyrhizobium japonicum 110 isatinate isatinate i.e. 2-(2-aminophenyl)-2-oxoacetate ?
3.5.2.20 isatin + H2O the enzyme is involved in the degradation of indole-3-acetic acid Neorhizobium galegae HAMBI 540 isatinate
-
 ?
3.5.2.20 isatin + H2O isatin i.e. 1H-indole-2,3-dione Neorhizobium galegae HAMBI 540 isatinate isatinate i.e. 2-(2-aminophenyl)-2-oxoacetate ?

Synonyms

EC Number Synonyms Comment Organism
3.5.2.20 isatin amidohydrolase
-
 Bradyrhizobium japonicum
3.5.2.20 isatin amidohydrolase
-
 Mesorhizobium loti
3.5.2.20 isatin amidohydrolase
-
 Neorhizobium galegae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.2.20 32
-
 assay at Bradyrhizobium japonicum
3.5.2.20 32
-
 assay at Mesorhizobium loti

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.2.20 9
-
 assay at Bradyrhizobium japonicum
3.5.2.20 9
-
 assay at Mesorhizobium loti

Expression

EC Number Organism Comment Expression
3.5.2.20 Mesorhizobium loti the enzyme is inducible (4-5-fold) by its substrate, isatin up

General Information

EC Number General Information Comment Organism
3.5.2.20 metabolism the enzyme is involved in the degradation of indole-3-acetic acid Bradyrhizobium japonicum
3.5.2.20 metabolism the enzyme is involved in the degradation of indole-3-acetic acid Mesorhizobium loti
3.5.2.20 metabolism the enzyme is involved in the degradation of indole-3-acetic acid Neorhizobium galegae