Literature summary extracted from
Yoon, G.M.; Kieber, J.J.
14-3-3 regulates 1-aminocyclopropane-1-carboxylate synthase protein turnover in Arabidopsis (2013), Plant Cell, 25, 1016-1028.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
4.4.1.14 |
coexpression of HA-tagged EOL2, HA-tagged isozyme ACS5, and increasing levels of HA-tagged 14-3-3omega protein in Arabidospis thaliana protoplasts, seedling phenotypes, overview |
Arabidopsis thaliana |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
4.4.1.14 |
additional information |
a transient expression system in Arabidopsis protoplasts is used to determine if the interaction with 14-3-3 protein increases the half-life of ACS5. Coexpression of HA-14-3-3v increases the half-life of myc-tagged ACS5 protein in this system |
Arabidopsis thaliana |
General Stability
EC Number |
General Stability |
Organism |
---|
4.4.1.14 |
14-3-3 proteins interact with multiple 1-aminocyclopropane-1-carboxylate synthase isoforms in Arabidopsis thaliana leading to stabilization of the isozymes |
Arabidopsis thaliana |
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
4.4.1.14 |
cytoplasm |
- |
Arabidopsis thaliana |
5737 |
- |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.4.1.14 |
Arabidopsis thaliana |
- |
- |
- |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
4.4.1.14 |
ACS |
- |
Arabidopsis thaliana |
General Information
EC Number |
General Information |
Comment |
Organism |
---|
4.4.1.14 |
metabolism |
the enzyme catalyzes the generally rate-limiting step in the biosynthesis of the phytohormone ethylene. 14-3-3 proteins exhibit a regulatory function in the pathway by reducing the degradation of 1-aminocyclopropane-1-carboxylate synthase through components of a CULLIN-3 E3 ubiquitin ligase, i.e. ethylene-overproducer 1-like proteins or ETO1/EOLs, that target a subset of the 1-aminocyclopropane-1-carboxylate synthase proteins for rapid degradation by the 26S proteasome. 14-3-3 protein positively regulates type-2 ACS protein stability by both increasing the turnover of the ETO1/EOL BTB E3 ligases that target type-2 ACS proteins and by an ETO1/EOL-independent mechanism. 14-3-3 protein promotes the degradation of ETO1/EOLs, likely via the 26S proteasome pathway |
Arabidopsis thaliana |
4.4.1.14 |
additional information |
14-3-3 proteins interact with multiple 1-aminocyclopropane-1-carboxylate synthase isoforms in Arabidopsis thaliana, 14-3-3 likely acts on all three classes of enzyme proteins |
Arabidopsis thaliana |