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Literature summary extracted from

  • Yang, C.Q.; Wu, X.M.; Ruan, J.X.; Hu, W.L.; Mao, Y.B.; Chen, X.Y.; Wang, L.J.
    Isolation and characterization of terpene synthases in cotton (Gossypium hirsutum) (2013), Phytochemistry, 96, 46-56.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.57 0.0289
-
 (2E,6E)-farnesyl diphosphate 30°C, pH not specified in the publication Gossypium hirsutum
4.2.3.104 0.0289
-
 (2E,6E)-farnesyl diphosphate 30°C, pH not specified in the publication Gossypium hirsutum
4.2.3.119 0.793
-
 geranyl diphosphate 30°C, pH not specified in the publication Gossypium hirsutum
4.2.3.120 0.793
-
 geranyl diphosphate 30°C, pH not specified in the publication Gossypium hirsutum

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.57 Gossypium hirsutum K7PRF2 isoform TPS1
-
4.2.3.104 Gossypium hirsutum K7PRF2 isoform TPS1
-
4.2.3.104 Gossypium hirsutum U5N1F1 isoform TPS2
-
4.2.3.119 Gossypium hirsutum U5N0S4 isoform TPS3
-
4.2.3.120 Gossypium hirsutum U5N0S4 isoform TPS3
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.57 cotyledon lowest expression level Gossypium hirsutum
-
4.2.3.57 hypocotyl
-
 Gossypium hirsutum
-
4.2.3.57 leaf
-
 Gossypium hirsutum
-
4.2.3.57 pericarp highest expression level Gossypium hirsutum
-
4.2.3.104 cotyledon lowest expression level Gossypium hirsutum
-
4.2.3.104 hypocotyl
-
 Gossypium hirsutum
-
4.2.3.104 leaf
-
 Gossypium hirsutum
-
4.2.3.104 leaf high expression Gossypium hirsutum
-
4.2.3.104 additional information expression is hardly detectable in other organs than leaf and pericarp Gossypium hirsutum
-
4.2.3.104 pericarp low expression Gossypium hirsutum
-
4.2.3.104 pericarp highest expression level Gossypium hirsutum
-
4.2.3.119 cotyledon
-
 Gossypium hirsutum
-
4.2.3.119 hypocotyl
-
 Gossypium hirsutum
-
4.2.3.119 leaf
-
 Gossypium hirsutum
-
4.2.3.119 pericarp
-
 Gossypium hirsutum
-
4.2.3.119 plant ovule
-
 Gossypium hirsutum
-
4.2.3.120 cotyledon
-
 Gossypium hirsutum
-
4.2.3.120 hypocotyl
-
 Gossypium hirsutum
-
4.2.3.120 leaf
-
 Gossypium hirsutum
-
4.2.3.120 pericarp
-
 Gossypium hirsutum
-
4.2.3.120 plant ovule
-
 Gossypium hirsutum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.57 (2E,6E)-farnesyl diphosphate
-
 Gossypium hirsutum beta-caryophyllene + diphosphate enzyme produces beta-caryophyllene and alpha-humulene in a ratio of 71:29 ?
4.2.3.104 (2E,6E)-farnesyl diphosphate
-
 Gossypium hirsutum alpha-humulene + diphosphate enzyme produces beta-caryophyllene and alpha-humulene in a ratio of 71:29 ?
4.2.3.104 4 (2E,6E)-farnesyl diphosphate
-
 Gossypium hirsutum guaia-1(10),11-diene + guaia-1(5),11-diene + alpha-humulene + beta-elemene + 4 diphosphate products are 55% guaia-1(10),11-diene, 14% guaia-1(5),11-diene, 10% alpha-humulene, 10% beta-elemene, 6% alloaromadendrene and 5% beta-caryophyllene ?
4.2.3.119 geranyl diphosphate
-
 Gossypium hirsutum alpha-pinene + diphosphate products are 77% alpha-pinene + 14.7% beta-pinene + 5.5 beta-phellandrene ?
4.2.3.120 geranyl diphosphate
-
 Gossypium hirsutum beta-pinene + diphosphate products are 77% alpha-pinene + 14.7% beta-pinene + 5.5 beta-phellandrene ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.57 TPS1
-
 Gossypium hirsutum
4.2.3.104 TPS1
-
 Gossypium hirsutum
4.2.3.104 TPS2
-
 Gossypium hirsutum
4.2.3.119 TPS3
-
 Gossypium hirsutum
4.2.3.120 TPS3
-
 Gossypium hirsutum

Expression

EC Number Organism Comment Expression
4.2.3.57 Gossypium hirsutum expression is induced upon wounding or methyljasmonate treatment up
4.2.3.104 Gossypium hirsutum expression is induced upon wounding or methyljasmonate treatment up
4.2.3.119 Gossypium hirsutum expression is induced upon wounding or methyljasmonate treatment up
4.2.3.120 Gossypium hirsutum expression is induced upon wounding or methyljasmonate treatment up

General Information

EC Number General Information Comment Organism
4.2.3.104 physiological function after tobacco rattlevirus-based virus induced gene silencing, beta-elemene, guaia-1(5),11-diene and guaia-1(10),11-diene are undetectable in suppressed leaves Gossypium hirsutum
4.2.3.119 physiological function in isoform TPS3-silenced plants, alpha-pinene, beta-pinene and beta-phellandrene contents decrease by more than 80%, whereas beta-myrcene is unchanged Gossypium hirsutum
4.2.3.120 physiological function in isoform TPS3-silenced plants, alpha-pinene, beta-pinene and beta-phellandrene contents decrease by more than 80%, whereas beta-myrcene is unchanged Gossypium hirsutum