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Literature summary extracted from

  • Greiner-Tollersrud, L.; Berg, T.; Stensland, H.M.; Evjen, G.; Greiner-Tollersrud, O.K.
    Bovine brain myelin glycerophosphocholine choline phosphodiesterase is an alkaline lysosphingomyelinase of the eNPP-family, regulated by lysosomal sorting (2013), Neurochem. Res., 38, 300-310.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.4.38 diagnostics the enzyme is a myelin marker enzyme, reflecting the quantity of myelin and mature oligodendrocytes Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.38 0.005
-
 lysosphingomyelin soluble isozyme, pH 8.0, 37°C Bos taurus
3.1.4.38 0.034
-
 4-nitrophenyl phosphocholine soluble isozyme, pH 8.0, 37°C Bos taurus
3.1.4.38 2
-
 lysophosphatidylcholine soluble isozyme, pH 8.0, 37°C Bos taurus
3.1.4.38 5
-
 sn-glycero-3-phosphocholine soluble isozyme, pH 8.0, 37°C Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.38 membrane two forms of brain GPC-Cpde, a membrane-linked, mGPC-Cpde, and a soluble, sGPC-Cpde Bos taurus 16020
-
3.1.4.38 additional information the truncated high mannose and bisected hybrid type glycans linked to N118 and N341 of the soluble isozyme sGPC-Cpde is a hallmark of glycans in lysosomal glycoproteins, subjected to GlcNAc-1-phosphorylation en route through the Golgi apparatus. The soluble isozyme sGPC-Cpde may originate from the lysosomes, suggesting that lysosomal sorting contributes to the level of membrane isozyme mGPC-Cpde on the myelin membrane Bos taurus
-
-
3.1.4.38 soluble two forms of brain GPC-Cpde, a membrane-linked, mGPC-Cpde, and a soluble, sGPC-Cpde. The enzyme is solubilised by C-terminal proteolysis, releasing the GPI-anchor, the resulting soluble isozyme sGPCCpde exists in two conformations, a homodimer joined by a disulfide bridge linking C414 from each monomer, and a monomer resulting from proteolysis N-terminally to this disulfide bond Bos taurus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.38 Co2+ activates Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.38 55000
-
 monomeric conformation of the soluble isozyme, gel filtration Bos taurus
3.1.4.38 110000
-
 dimeric conformation of the soluble isozyme, gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.38 lysophosphatidylcholine + H2O Bos taurus
-
 choline + 2-lysophosphatidate
-
 ?
3.1.4.38 lysophosphatidylcholine + H2O Bos taurus Norwegian Red Cattle
-
 choline + 2-lysophosphatidate
-
 ?
3.1.4.38 lysosphingomyelin + H2O Bos taurus best substrate of the soluble isozyme sGPC-Cpde ?
-
 ?
3.1.4.38 lysosphingomyelin + H2O Bos taurus Norwegian Red Cattle best substrate of the soluble isozyme sGPC-Cpde ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.38 Bos taurus
-
-
-
3.1.4.38 Bos taurus Norwegian Red Cattle
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.4.38 glycoprotein truncated high mannose and bisected hybrid type glycans are linked to N118 and N341 of the soluble isozyme sGPC-Cpde, a hallmark of glycans in lysosomal glycoproteins, subjected to GlcNAc-1-phosphorylation en route through the Golgi apparatus. All the four N-glycosylation sites N100KS, N118GS, N341ST and N406GS in the soluble isozyme sGPC-Cpde are occupied, mainly with endo H-sensitive glycans, QTOF-MS-MS analysis Bos taurus
3.1.4.38 phosphoprotein truncated high mannose and bisected hybrid type glycans are linked to N118 and N341 of the soluble isozyme sGPC-Cpde, a hallmark of glycans in lysosomal glycoproteins, subjected to GlcNAc-1-phosphorylation en route through the Golgi apparatus Bos taurus
3.1.4.38 proteolytic modification two forms of brain GPC-Cpde, a membrane-linked, mGPC-Cpde, and a soluble, sGPC-Cpde. The enzyme is solubilised by C-terminal proteolysis, releasing the GPI-anchor, the resulting soluble isozyme sGPCCpde exists as two conformations, a homodimer joined by a disulfide bridge linking C414 from each monomer, and a monomer resulting from proteolysis N-terminally to this disulfide bond Bos taurus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.38 native enzyme from brain 396fold by heat treatment at 60°C, concanavalin A affinity chromatography, and hydroxylapatite chromatography, followed by dialysis, anion exchange chromatography and gel filtration Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.38 brain two forms of brain GPC-Cpde, a membrane-linked, mGPC-Cpde, and a soluble, sGPC-Cpde Bos taurus
-
3.1.4.38 kidney
-
 Bos taurus
-
3.1.4.38 myelin membrane membrane-linked isozyme mGPC-Cpde Bos taurus
-
3.1.4.38 oligodendrocyte the enzyme is expressed on the myelin membrane Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.38 3.2
-
 purified native soluble isozyme, pH 8.0, 37°C, substrate 4-nitrophenyl phosphocholine Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.38 4-nitrophenyl phosphocholine + H2O
-
 Bos taurus 4-nitrophenol + phosphocholine
-
 ?
3.1.4.38 lysophosphatidylcholine + H2O first leaving group is acylglycerol, second is phosphocholine Bos taurus ?
-
 ?
3.1.4.38 lysophosphatidylcholine + H2O first leaving group is acylglycerol, second is phosphocholine Bos taurus Norwegian Red Cattle ?
-
 ?
3.1.4.38 lysophosphatidylcholine + H2O
-
 Bos taurus choline + 2-lysophosphatidate
-
 ?
3.1.4.38 lysophosphatidylcholine + H2O
-
 Bos taurus Norwegian Red Cattle choline + 2-lysophosphatidate
-
 ?
3.1.4.38 lysosphingomyelin + H2O best substrate of the soluble isozyme sGPC-Cpde Bos taurus ?
-
 ?
3.1.4.38 lysosphingomyelin + H2O best substrate of the soluble isozyme sGPC-Cpde. First leaving group is sphingosin, second is phosphocholine Bos taurus ?
-
 ?
3.1.4.38 lysosphingomyelin + H2O best substrate of the soluble isozyme sGPC-Cpde Bos taurus Norwegian Red Cattle ?
-
 ?
3.1.4.38 lysosphingomyelin + H2O best substrate of the soluble isozyme sGPC-Cpde. First leaving group is sphingosin, second is phosphocholine Bos taurus Norwegian Red Cattle ?
-
 ?
3.1.4.38 additional information the soluble isozyme sGPC-Cpde is specific for lysosphingomyelin, displaying 1 to 2 orders of magnitude higher catalytic activity than towards sn-glycero-3-phosphocholine and lysophosphatidylcholine Bos taurus ?
-
 ?
3.1.4.38 additional information the soluble isozyme sGPC-Cpde is specific for lysosphingomyelin, displaying 1 to 2 orders of magnitude higher catalytic activity than towards sn-glycero-3-phosphocholine and lysophosphatidylcholine Bos taurus Norwegian Red Cattle ?
-
 ?
3.1.4.38 sn-glycero-3-phosphocholine + H2O first leaving group is glycerol, second is phosphocholine Bos taurus glycerol + phosphocholine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.38 dimer dimeric conformation of the soluble isozyme, 2 * 55000, SDS-PAGE Bos taurus
3.1.4.38 monomer monomeric conformation of the soluble isozyme, 1 * 55000, SDS-PAGE Bos taurus

Synonyms

EC Number Synonyms Comment Organism
3.1.4.38 alkaline lysosphingomyelinase
-
 Bos taurus
3.1.4.38 glycerophosphocholine choline phosphodiesterase
-
 Bos taurus
3.1.4.38 glycosylphosphatidylinositol-anchored alkaline hydrolase
-
 Bos taurus
3.1.4.38 GPC-Cpde
-
 Bos taurus
3.1.4.38 GPI-anchored alkaline hydrolase
-
 Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.4.38 37
-
 assay at Bos taurus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.4.38 1
-
 sn-glycero-3-phosphocholine soluble isozyme, pH 8.0, 37°C Bos taurus
3.1.4.38 2
-
 4-nitrophenyl phosphocholine soluble isozyme, pH 8.0, 37°C Bos taurus
3.1.4.38 2.5
-
 lysophosphatidylcholine soluble isozyme, pH 8.0, 37°C Bos taurus
3.1.4.38 65
-
 lysosphingomyelin soluble isozyme, pH 8.0, 37°C Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.38 8
-
 assay at Bos taurus

General Information

EC Number General Information Comment Organism
3.1.4.38 evolution the soluble isozyme belongs to the eNPP enzyme family Bos taurus
3.1.4.38 physiological function the soluble isozyme may function in the sphingomyelin signaling, rather than in the homeostasis of acylglycerophosphocholine metabolites Bos taurus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.4.38 1
-
 sn-glycero-3-phosphocholine soluble isozyme, pH 8.0, 37°C Bos taurus
3.1.4.38 10
-
 lysophosphatidylcholine soluble isozyme, pH 8.0, 37°C Bos taurus
3.1.4.38 70
-
 4-nitrophenyl phosphocholine soluble isozyme, pH 8.0, 37°C Bos taurus
3.1.4.38 200
-
 lysosphingomyelin soluble isozyme, pH 8.0, 37°C Bos taurus