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Literature summary extracted from

  • Nguyen, C.; Haushalter, R.; Lee, D.; Markwick, P.; Bruegger, J.; Caldara-Festin, G.; Finzel, K.; Jackson, D.; Ishikawa, F.; O'Dowd, B.; McCammon, J.; Opella, S.; Tsai, S.; Burkart, M.
    Trapping the dynamic acyl carrier protein in fatty acid biosynthesis (2014), Nature, 505, 427-431.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.59 expressed in Escherichia coli BL21(DE3) cells Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.1.59 sitting drop vapor diffusion method, using 10 mM sodium phosphate (pH 8.0), 350 mM sodium acetate, 1 M LiCl and 35% (w/v) PEG3350 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.59 (3R)-3-hydroxydecanoyl-[acyl-carrier protein] Escherichia coli
-
 2-decenoyl-[acyl-carrier protein] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.59 Escherichia coli P0A6Q3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.59 Ni-affinity column chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.59 (3R)-3-hydroxydecanoyl-[acyl-carrier protein]
-
 Escherichia coli 2-decenoyl-[acyl-carrier protein] + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.59 3-hydroxyacyl-ACP dehydratase
-
 Escherichia coli