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Literature summary extracted from

  • Belaunzaran, M.L.; Wilkowsky, S.E.; Lammel, E.M.; Gimenez, G.; Bott, E.; Barbieri, M.A.; de Isola, E.L.
    Phospholipase A1: a novel virulence factor in Trypanosoma cruzi (2013), Mol. Biochem. Parasitol., 187, 77-86.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.32 DNA and amino acid equence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 (DE3) pLysS, the enzyme is mainly localized in inclusion bodies Trypanosoma cruzi

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.32 membrane the enzyme is a membrane-bound/secreted enzyme in the infective stages of Trypanosoma cruzi Trypanosoma cruzi 16020
-
3.1.1.32 additional information immunohistochemic analysis of subcellular localization Trypanosoma cruzi
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.32 38000
-
 x * 38000, SDS-PAGE Trypanosoma cruzi

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.32 Trypanosoma cruzi H2EIY3 strains Cvd and RA (lethal) and K98 (non-lethal)
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.32 soluble portion of recombinant His-tagged enzyme from Escherichia coli strain BL21 (DE3) pLysS by nickel affinity chromatography Trypanosoma cruzi

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.32 amastigote infective Trypanosoma cruzi
-
3.1.1.32 epimastigote
-
 Trypanosoma cruzi
-
3.1.1.32 trypomastigote
-
 Trypanosoma cruzi
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.32 1,2-dipalmitoyl-phosphatidylcholine + H2O substrate from Glycine max Trypanosoma cruzi 2-palmitoyl-sn-glycerophosphocholine + palmitate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.32 ? x * 38000, SDS-PAGE Trypanosoma cruzi

Synonyms

EC Number Synonyms Comment Organism
3.1.1.32 PLA1
-
 Trypanosoma cruzi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.32 37
-
 assay at Trypanosoma cruzi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.32 4.7
-
 assay at Trypanosoma cruzi

Expression

EC Number Organism Comment Expression
3.1.1.32 Trypanosoma cruzi increased levels of PLA1 expression in the infective amastigotes and trypomastigotes compared to the non-infective epimastigotes of lethal RA strain up

General Information

EC Number General Information Comment Organism
3.1.1.32 physiological function lysophospholipase activity in live Tryypanosoma cruzi parasites contributes to self protection against toxic compounds generated by phospholipase A1 activity Trypanosoma cruzi