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Literature summary extracted from
- Steinernema glaseri surface enolase: molecular cloning, biological characterization, and role in host immune suppression (2012), Mol. Biochem. Parasitol., 185, 89-98.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.1.11 | expressed in Escherichia coli BL21(DE3) cells | Steinernema glaseri |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.11 | 47000 | - |
x * 47000, SDS-PAGE | Steinernema glaseri |
| 4.2.1.11 | 47200 | - |
x * 47200, calculated from amino acid sequence | Steinernema glaseri |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.11 | 2-phospho-D-glycerate | Steinernema glaseri | - |
phosphoenolpyruvate + H2O | - |
? |  |
| 4.2.1.11 | 2-phospho-D-glycerate | Steinernema glaseri NC | - |
phosphoenolpyruvate + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.11 | Steinernema glaseri | B8Q027 | - |
- |
| 4.2.1.11 | Steinernema glaseri NC | B8Q027 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.11 | GSTrap FF column chromatography and Superdex 200 gel filtration | Steinernema glaseri |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 4.2.1.11 | cuticle | - |
Steinernema glaseri | - |
| 4.2.1.11 | hemolymph | - |
Steinernema glaseri | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.11 | 2-phospho-D-glycerate | - |
Steinernema glaseri | phosphoenolpyruvate + H2O | - |
? | |
| 4.2.1.11 | 2-phospho-D-glycerate | - |
Steinernema glaseri NC | phosphoenolpyruvate + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.1.11 | ? | x * 47000, SDS-PAGE | Steinernema glaseri |
| 4.2.1.11 | ? | x * 47200, calculated from amino acid sequence | Steinernema glaseri |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.2.1.11 | ENOL | - |
Steinernema glaseri |
| 4.2.1.11 | enolase | - |
Steinernema glaseri |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 4.2.1.11 | Steinernema glaseri | calculated from amino acid sequence | - |
5.6 |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 4.2.1.11 | physiological function | the enzyme promotes infection by Xenorhabdus poinarii and Metarhizium anisopliae | Steinernema glaseri |
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Release 2023.1 (January 2023)
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