BRENDA - Enzyme Database

Steinernema glaseri surface enolase: molecular cloning, biological characterization, and role in host immune suppression

Liu, H.; Zeng, H.; Yao, Q.; Yuan, J.; Zhang, Y.; Qiu, D.; Yang, X.; Yang, H.; Liu, Z.; Mol. Biochem. Parasitol. 185, 89-98 (2012)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.11
expressed in Escherichia coli BL21(DE3) cells
Steinernema glaseri
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
47000
-
x * 47000, SDS-PAGE
Steinernema glaseri
4.2.1.11
47200
-
x * 47200, calculated from amino acid sequence
Steinernema glaseri
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Steinernema glaseri
-
phosphoenolpyruvate + H2O
-
-
?
4.2.1.11
2-phospho-D-glycerate
Steinernema glaseri NC
-
phosphoenolpyruvate + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Steinernema glaseri
B8Q027
-
-
4.2.1.11
Steinernema glaseri NC
B8Q027
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
GSTrap FF column chromatography and Superdex 200 gel filtration
Steinernema glaseri
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
cuticle
-
Steinernema glaseri
-
4.2.1.11
hemolymph
-
Steinernema glaseri
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
730353
Steinernema glaseri
phosphoenolpyruvate + H2O
-
-
-
?
4.2.1.11
2-phospho-D-glycerate
-
730353
Steinernema glaseri NC
phosphoenolpyruvate + H2O
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.11
?
x * 47000, SDS-PAGE; x * 47200, calculated from amino acid sequence
Steinernema glaseri
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
4.2.1.11
Steinernema glaseri
calculated from amino acid sequence
-
5.6
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
expressed in Escherichia coli BL21(DE3) cells
Steinernema glaseri
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
47000
-
x * 47000, SDS-PAGE
Steinernema glaseri
4.2.1.11
47200
-
x * 47200, calculated from amino acid sequence
Steinernema glaseri
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Steinernema glaseri
-
phosphoenolpyruvate + H2O
-
-
?
4.2.1.11
2-phospho-D-glycerate
Steinernema glaseri NC
-
phosphoenolpyruvate + H2O
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
GSTrap FF column chromatography and Superdex 200 gel filtration
Steinernema glaseri
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
cuticle
-
Steinernema glaseri
-
4.2.1.11
hemolymph
-
Steinernema glaseri
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
730353
Steinernema glaseri
phosphoenolpyruvate + H2O
-
-
-
?
4.2.1.11
2-phospho-D-glycerate
-
730353
Steinernema glaseri NC
phosphoenolpyruvate + H2O
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.11
?
x * 47000, SDS-PAGE; x * 47200, calculated from amino acid sequence
Steinernema glaseri
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
4.2.1.11
Steinernema glaseri
calculated from amino acid sequence
-
5.6
General Information
EC Number
General Information
Commentary
Organism
4.2.1.11
physiological function
the enzyme promotes infection by Xenorhabdus poinarii and Metarhizium anisopliae
Steinernema glaseri
General Information (protein specific)
EC Number
General Information
Commentary
Organism
4.2.1.11
physiological function
the enzyme promotes infection by Xenorhabdus poinarii and Metarhizium anisopliae
Steinernema glaseri