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Literature summary extracted from

  • Hu, Y.; Meng, J.; Shi, C.; Hervin, K.; Fratamico, P.M.; Shi, X.
    Characterization and comparative analysis of a second thermonuclease from Staphylococcus aureus (2013), Microbiol. Res., 168, 174-182.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.31.1 2-mercaptoethanol
-
 Staphylococcus aureus
3.1.31.1 dithiothreitol
-
 Staphylococcus aureus
3.1.31.1 DTT 163.4% of initial isozyme Nuc1 activity at 1 mM Staphylococcus aureus
3.1.31.1 Triton X-100
-
 Staphylococcus aureus
3.1.31.1 Triton X-100 210.5% of initial isozyme Nuc1 activity at 1% Staphylococcus aureus
3.1.31.1 Tween-20
-
 Staphylococcus aureus
3.1.31.1 Urea
-
 Staphylococcus aureus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.31.1 gene nuc1, sequence comparison and phylogenetic analysis, functional recombinant expression in Escherichia coli strain BL21(DE3) Staphylococcus aureus
3.1.31.1 gene nuc2, sequence comparison and phylogenetic analysis, functional recombinant expression in Escherichia coli strain BL21(DE3) Staphylococcus aureus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.31.1 additional information generation of a nuc1/nuc2 double deletion mutant Staphylococcus aureus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.31.1 Co2+ isozyme Nuc1 shows 79% activity at 5 mM concentration Staphylococcus aureus
3.1.31.1 EDTA
-
 Staphylococcus aureus
3.1.31.1 Mn2+ isozyme Nuc1 activity drops sharply at 5 mM concentration; isozyme Nuc2 activity decreases with an increase in Mn2+ concentration Staphylococcus aureus
3.1.31.1 additional information Nuc2 shows weaker activity, lower thermostability and different sensitivity to Zn2+ and Mn2+, and in the presence of EDTA or SDS compared with Nuc1, which is consistent with differences in the sequence pattern and structure predicted; Nuc2 shows weaker activity, lower thermostability and different sensitivity to Zn2+ and Mn2+, and in the presence of EDTA or SDS compared with Nuc1, which is consistent with differences in the sequence pattern and structure predicted Staphylococcus aureus
3.1.31.1 SDS
-
 Staphylococcus aureus
3.1.31.1 Zn2+ isozyme Nuc1 activity drops sharply at 5 mM concentration; isozyme Nuc2 activity decreases with an increase in Zn2+ concentration Staphylococcus aureus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.31.1 extracellular
-
 Staphylococcus aureus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.31.1 Ca2+ activates to 120% of initial activity at 0.05 mM Staphylococcus aureus
3.1.31.1 Co2+ activates slightly at 5 mM Staphylococcus aureus
3.1.31.1 Mg2+ activates to 120% of initial activity at 0.5 mM Staphylococcus aureus
3.1.31.1 additional information isozyme Nuc1 only exhibits a very small increase in thermonuclease activity in the presence of Ca2+ (0.05 mM), Mg2+ (0.05 mM), Co2+ (0.5 mM) and Ni2+ (0.05 mM) Staphylococcus aureus
3.1.31.1 Ni2+ activates slightly at 0.05 mM Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
3.1.31.1 Staphylococcus aureus A0A0H3JPX6 gene nuc2; strain RN4220, gene nuc2
-
3.1.31.1 Staphylococcus aureus Q7A6P2 gene nuc1; strain RN4220, gene nuc1
-
3.1.31.1 Staphylococcus aureus N315 Q7A6P2 gene nuc1; strain RN4220, gene nuc1
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.31.1 additional information isozyme Nuc1 is active with genomic DNA extracted from Staphylococcus aureus, Listeria monocytogenes, and Salmonella, herring sperm DNA, plasmid DNA pNucc from Staphylococcus aureus and pBR122 from Escherichia coli, and RNA from Staphylococcus aureus, substrate specificity of the recombinant nuclease, overview Staphylococcus aureus ?
-
 ?
3.1.31.1 additional information isozyme Nuc2 is active with genomic DNA extracted from Staphylococcus aureus, Listeria monocytogenes, and Salmonella, herring sperm DNA, plasmid DNA pNucc from Staphylococcus aureus and pBR122 from Escherichia coli, and RNA from Staphylococcus aureus, substrate specificity of the recombinant nuclease, overview Staphylococcus aureus ?
-
 ?
3.1.31.1 additional information isozyme Nuc1 is active with genomic DNA extracted from Staphylococcus aureus, Listeria monocytogenes, and Salmonella, herring sperm DNA, plasmid DNA pNucc from Staphylococcus aureus and pBR122 from Escherichia coli, and RNA from Staphylococcus aureus, substrate specificity of the recombinant nuclease, overview Staphylococcus aureus N315 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.31.1 More structure comparison of isozyme Nuc1 and Nuc2, overview Staphylococcus aureus

Synonyms

EC Number Synonyms Comment Organism
3.1.31.1 NUC1
-
 Staphylococcus aureus
3.1.31.1 Nuc2
-
 Staphylococcus aureus
3.1.31.1 staphylococcal nuclease
-
 Staphylococcus aureus
3.1.31.1 thermonuclease
-
 Staphylococcus aureus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.31.1 50
-
 recombinant enzyme Staphylococcus aureus
3.1.31.1 55
-
-
 Staphylococcus aureus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.31.1 20 80 effects of temperature on thermonuclease activity of isozymes Nuc1 and Nuc2, profile overview Staphylococcus aureus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.31.1 additional information
-
 thermostable enzyme Staphylococcus aureus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.31.1 9 10
-
 Staphylococcus aureus
3.1.31.1 10
-
 recombinant enzyme Staphylococcus aureus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.31.1 3 10 effects of pH on thermonuclease activity of isozymes Nuc1 and Nuc2, profile overview Staphylococcus aureus

General Information

EC Number General Information Comment Organism
3.1.31.1 physiological function major role for isozyme Nuc1 in terms of thermonuclease activity Staphylococcus aureus